PFDN6 / HKE2 (1-129, His-tag) - AR09906PU-N

Product Description for PFDN6 / HKE2

PFDN6 / HKE2.
Presentation: Purified

Immunogen	Swiss Prot Num: O15212
Purity	> 95 %
Background	PFDN6, also known as HKE2 or PFD6, is a subunit of the heteromeric prefoldin complex that chaperones nascent actin and alpha- and beta-tubulin chains pending their transfer to the cytosolic chaperonin containing TCP1 (CCT) complex. It binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it and binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.
Concentration	0.5 mg/ml (determined by BCA assay)
General Readings	Sowa ME., et al. (2009) Cell. 138(2):389-403. Simons CT., et al. (2004) J Biol Chem. 279(6):4196-203.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human PFDN6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MAELIQKKLQ GEVEKYQQLQ KDLSKSMSGR QKLEAQLTEN NIVKEELALL DGSNVVFKLL GPVLVKQELG EARATVGKRL DYITAEIKRY ESQLRDLERQ SEQQRETLAQ LQQEFQRAQA AKAGAPGKA` Molecular weight: (149aa) confirmed by MALDI-TOF
Format	Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol Purity: >95% State: Liquid purified protein Purified