AR09913PU-L NECAP2 (1-263, His-tag)

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0.5 mg / €820.00
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NECAP2

AR09913PU-L

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  • AR09913PU-L

Product Description for NECAP2

NECAP2.
Presentation: Purified

Properties for NECAP2

Product Category Proteins & Growth Factors
Quantity 0.5 mg
Synonyms Adaptin ear-binding coat-associated protein 2
Presentation Purified
Molecular weight 30.5 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9NVZ3
Purity > 85 %
Background NECAP2, also known as adaptin ear-binding coat-associated protein 2, is a essential protein paralogues for clathrin-mediated membrane trafficking that are enriched in CCV coats. This protein colocalizes with AP-2 at the plasma membrane by binding AP-2s α-ear domain, and interacts with AP-1, AP-2 and several GAE domain proteins termed GGA1, GGA2 and GGA3. NECAP2 is Involved in endocytosis.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Miller GJ., et al. (2003) Nat Struct Biol. 10(8):599-606.
Mattera R., et al. (2004) J Biol Chem. 279(9):8018-28.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human NECAP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW GDFTKSTGST SSQTQPGTGW VQF
Molecular weight:
(283 aa) confirmed by MALDI-TOF
Format
Buffer System:
20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl
Purity:
>85%
State:
Liquid purified protein
Purified
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