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DDX0010A488-50 CD301 / CLEC10A antibody

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Mouse anti Human CD301 / CLEC10A 125A10.03

DDX0010A488-50

Product Description for CD301 / CLEC10A

Mouse anti Human CD301 / CLEC10A 125A10.03.
Presentation: Alexa Fluor 488
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for CD301 / CLEC10A

Product Category Primary Antibodies
Quantity 50 µg
Synonyms C-type lectin domain family 10 member A, C-type lectin superfamily member 14, CLECSF13, CLECSF14, HML, Macrophage lectin 2
Presentation Alexa Fluor 488
Reactivity Hu
Applications F, ICC/IF
Clonality Monoclonal
Clone 125A10.03
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q8IUN9
Immunogen:
human ASGPR Ig fusion protein
GeneID:
10462
Application Surface and intracyto Flow Cytometry, IF
Background ASGPR/MGL (asialoglycoprotein receptor/ macrophage galactose lectin receptor) / CD301 is a Ca2+ dependent type II transmembrane lectin. In search for genes specifically expressed by dendritic cells (DC), we have cloned several cDNAs encoding different forms of asialoglycoprotein receptor (ASGPR), that were expressed in eukaryotic cells for use as immunogens. Immunoprecipitation from DCs using anti-DCASGPR mAb yields a major 40-kDa protein. mAb 125A10.03 recognized the ASGPR isoform expressed by DC but not the isoform expressed by monocytes. This antibody stained CD14+derived DCs obtained from CD34+ progenitors, but not the CD1a+derived subset. Accordingly, both monocyte-derived DCs and tonsillar interstitial-type DCs express DC-ASGPR protein, while Langerhans-type cells do not. DC-ASGPR is a feature of immaturity, as its expression is lost upon CD40L activation. In agreement with the presence of tyrosine-based and dileucine motifs in the intracytoplasmic domain, mAb against DC-ASGPR is rapidly internalized by DCs at 37°C. Finally, intracellular DC-ASGPR is localized in early endosomes, suggesting that the receptor recycles to the cell surface following internalization of ligand. (Valladeau J et al, 2001, J. Immunol., 167: 5767).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Valladeau J, Duvert-Frances V, Pin JJ, Kleijmeer MJ, Ait-Yahia S, Ravel O, et al. Immature human dendritic cells express asialoglycoprotein receptor isoforms for efficient receptor-mediated endocytosis. J Immunol. 2001 Nov 15;167(10):5767-74. PubMed PMID: 11698450.
  2. Napoletano C, Zizzari IG, Rughetti A, Rahimi H, Irimura T, Clausen H, et al. Targeting of macrophage galactose-type C-type lectin (MGL) induces DC signaling and activation. Eur J Immunol. 2012 Apr;42(4):936-45. doi: 10.1002/eji.201142086. PubMed PMID: 22531918.
  3. Debeer S, Le Luduec JB, Kaiserlian D, Laurent P, Nicolas JF, Dubois B, et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol. 2013 Jul-Aug;23(4):456-66. doi: 10.1684/ejd.2013.2060. PubMed PMID: 24047577.
  4. l. G. Zizzari et al. Seasonal modulation of the C-type lectin MGL on human DCs. Open Journal of Immunology 3 (2013) 218-220. http://dx.doi.org/10.4236/oji.2013.34027.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Alexa Fluor 488
State:
100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
Specificity
Specificity:
human ASGPR/MGL
Gene ID 10462

Accessory Products

Proteins and/or Positive Controls

Proteins for CD301 / CLEC10A (7 products)

Catalog No. Species Pres. Purity   Source  

CD301 / CLEC10A (transcript variant 2)
CD301 / CLEC10A Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD301 / CLEC10A (transcript variant 2)
CD301 / CLEC10A Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

CD301 / CLEC10A
CD301 / CLEC10A Human
  Abnova Taiwan Corp.

CD301 / CLEC10A
CD301 / CLEC10A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD301 / CLEC10A
CD301 / CLEC10A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD301 / CLEC10A
CD301 / CLEC10A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD301 / CLEC10A
CD301 / CLEC10A Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

Positive controls for CD301 / CLEC10A (5 products)

Catalog No. Species Pres. Purity   Source  

CD301 Lysate
Western Blot: CD301 Lysate [NBL1-09254] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CLEC10A
  Novus Biologicals Inc.

CLEC10A 293T Cell Transient Overexpression Lysate(Denatured)
CLEC10A 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CLEC10A overexpression lysate
CLEC10A overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CLEC10A overexpression lysate
CLEC10A overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

CLEC10A overexpression lysate
CLEC10A overexpression lysate
0.1 mg / €315.00
  OriGene Technologies, Inc.

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Click here to see all secondary antibodies for CD301 / CLEC10A
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