discontinued

DDX0072P-50 BETVIA antibody

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Human anti BETVIA H.G11A9F3.03

Product Description for BETVIA

Human anti BETVIA H.G11A9F3.03.
Presentation: Purified
Product is tested for Enzyme Immunoassay.

Properties for BETVIA

Product Category Primary Antibodies
Quantity 50 µg
Synonyms Allergen Bet v I-A, BETVI, Major pollen allergen Bet v 1-A
Presentation Purified
Reactivity Pollen
Applications E
Clonality Monoclonal
Clone H.G11A9F3.03
Host Human
Isotype IgG4
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
P15494
Immunogen:
human B cells immortalized from a birch pollen allergic patient
Application ELISA (coating), IP. 
Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
Background Birch pollen allergy is a frequent pathology with IgE reactivity against Betv1 the major birch pollen allergen. Human monoclonal antibodies secreting B cells have been cloned from PBMC of allergic patients. These high affinity human monoclonal antibodies raised against BetV1 inhibit the binding of IgE to nitrocellulose-blotted Bet v1 and inhibit histamine release by sensitized basophils. (Visco V. et al, J. Immunol., 1996 ; 157: 956-962 ; Denépoux S et al, FEBS Lett. 2000 Jan 7;465(1):39-46.)
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Visco V, Dolecek C, Denépoux S, Le Mao J, Guret C, Rousset F, et al. Human IgG monoclonal antibodies that modulate the binding of specific IgE to birch pollen Bet v 1. J Immunol. 1996 Jul 15;157(2):956-62. PubMed PMID: 8752951.
  2. Denépoux S, Eibensteiner PB, Steinberger P, Vrtala S, Visco V, Weyer A, et al. Molecular characterization of human IgG monoclonal antibodies specific for the major birch pollen allergen Bet v 1. Anti-allergen IgG can enhance the anaphylactic reaction. FEBS Lett. 2000 Jan 7;465(1):39-46. PubMed PMID: 10620703.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Purification:
QMA Hyper-D Ion-exchange chromatography
State:
100 μg in 200 μl Tris-NaCl pH 8
Purified
Specificity
Specificity:
birch pollen Bet v1
Cross-reactivity: large panel of tree’s pollen

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