discontinued

DDX0080P-50 CD1a antibody

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Mouse anti Human CD1a 214A9.01

DDX0080P-50

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  • DDX0080P-50
  • DDX0080P-50

Product Description for CD1a

Mouse anti Human CD1a 214A9.01.
Presentation: Purified
Product is tested for Frozen Sections, Western blot / Immunoblot, Flow Cytometry.

Properties for CD1a

Product Category Primary Antibodies
Quantity 50 µg
Synonyms T-cell surface antigen T6/Leu-6, T-cell surface glycoprotein CD1a, T6/Leu-6, hTa1, hTa1 thymocyte antigen
Presentation Purified
Reactivity Hu
Applications C, F, WB
Clonality Monoclonal
Clone 214A9.01
Host Mouse
Isotype IgM
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
P06126
Immunogen:
in vitro derived human DCs (GMCSF + TNFα)
GeneID:
909
Isotype control SM13P
Application Flow Cytometry, IHC cryosection, WB.
Background CD1 family molecules have a homologous structure to MHC class I. They are monomorphic proteins of 50 kDa non-covalently associated with β2-microglobulin. Human CD1a molecules are divided in 2 groups. Group 1 includes CD1a, CD1b, and CD1c, while group 2 consists of the CD1d molecule. CD1a is mainly expressed at the cell-surface and in recycling vesicles. CD1a occurs in Birbeck’s granules of Langerhans cells and plays a role in presentation of non-peptide antigens. CD1a is expressed in vitro on CD34+ generated DCs and on monocyte-derived DCs.
(Burdin N. et al, 1999 ; Curr. Opin. Immunol., 11:326-31 ; Valladeau J. et al, 1999; Immunity, 12:71-81)
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Valladeau J, Duvert-Frances V, Pin JJ, Dezutter-Dambuyant C, Vincent C, Massacrier C, et al. The monoclonal antibody DCGM4 recognizes Langerin, a protein specific of Langerhans cells, and is rapidly internalized from the cell surface. Eur J Immunol. 1999 Sep;29(9):2695-704. PubMed PMID: 10508244.
  2. Valladeau J, Ravel O, Dezutter-Dambuyant C, Moore K, Kleijmeer M, Liu Y, et al. Langerin, a novel C-type lectin specific to Langerhans cells, is an endocytic receptor that induces the formation of Birbeck granules. Immunity. 2000 Jan;12(1):71-81. PubMed PMID: 10661407.
  3. Poulin LF, Salio M, Griessinger E, Anjos-Afonso F, Craciun L, Chen JL, et al. Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells. J Exp Med. 2010 Jun 7;207(6):1261-71. doi: 10.1084/jem.20092618. Epub 2010 May 17. PubMed PMID: 20479117. (Free PMC Article available, 6 images available)
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Format
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8
State:
Purified
Specificity
Specificity:
human CD1a (epitope in extracellular domain).
Gene ID 909

Accessory Products

Proteins and/or Positive Controls

Proteins for CD1a (3 products)

Catalog No. Species Pres. Purity   Source  

CD1a

CD1a Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD1a

CD1a Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD1a

CD1a Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for CD1a (1 products)

Catalog No. Species Pres. Purity   Source  

CD1A overexpression lysate

CD1A overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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