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DDX0131A488-50 CD40 antibody

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Mouse anti Human CD40 mAb89

DDX0131A488-50

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  • DDX0131A488-50
  • DDX0131A488-50

Product Description for CD40

Mouse anti Human CD40 mAb89.
Presentation: Alexa Fluor 488
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for CD40

Product Category Primary Antibodies
Quantity 50 µg
Synonyms B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5
Presentation Alexa Fluor 488
Reactivity Hu
Applications F, ICC/IF
Clonality Monoclonal
Clone mAb89
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
P25942
Immunogen:
anti-μ activated tonsillar human B cells
GeneID:
958
Application FACS surface, ImmunoFluorescence
Background CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40L (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40, predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.
(Vallé A. et al, 1989; Eur. J. Immunol., 19: 1463-1469; O’ Sullivan B. et al, 2003; Crit. Rev. Immunol., 23: 83-107)
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Björck P, Braesch-Andersen S, Paulie S. Antibodies to distinct epitopes on the CD40 molecule co-operate in stimulation and can be used for the detection of soluble CD40. Immunology. 1994 Nov;83(3):430-7. PubMed PMID: 7530692. (Free PMC Article available)
  2. Björck P, Paulie S. CD40 antibodies defining distinct epitopes display qualitative differences in their induction of B-cell differentiation. Immunology. 1996 Feb;87(2):291-5. PubMed PMID: 8698393. (Free PMC Article available)
  3. de Saint-Vis B, Vincent J, Vandenabeele S, Vanbervliet B, Pin JJ, Aït-Yahia S, et al. A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment. Immunity. 1998 Sep;9(3):325-36. PubMed PMID: 9768752.
  4. Bates EE, Fournier N, Garcia E, Valladeau J, Durand I, Pin JJ, et al. APCs express DCIR, a novel C-type lectin surface receptor containing an immunoreceptor tyrosine-based inhibitory motif. J Immunol. 1999 Aug 15;163(4):1973-83. PubMed PMID: 10438934.
  5. Henn V, Steinbach S, Büchner K, Presek P, Kroczek RA. The inflammatory action of CD40 ligand (CD154) expressed on activated human platelets is temporally limited by coexpressed CD40. Blood. 2001 Aug 15;98(4):1047-54. PubMed PMID: 11493450.
  6. Tong AW, Papayoti MH, Netto G, Armstrong DT, Ordonez G, Lawson JM, et al. Growth-inhibitory effects of CD40 ligand (CD154) and its endogenous expression in human breast cancer. Clin Cancer Res. 2001 Mar;7(3):691-703. PubMed PMID: 11297266.
  7. Valladeau J, Duvert-Frances V, Pin JJ, Kleijmeer MJ, Ait-Yahia S, Ravel O, et al. Immature human dendritic cells express asialoglycoprotein receptor isoforms for efficient receptor-mediated endocytosis. J Immunol. 2001 Nov 15;167(10):5767-74. PubMed PMID: 11698450.
  8. Contin C, Pitard V, Itai T, Nagata S, Moreau JF, Déchanet-Merville J. Membrane-anchored CD40 is processed by the tumor necrosis factor-alpha-converting enzyme. Implications for CD40 signaling. J Biol Chem. 2003 Aug 29;278(35):32801-9. Epub 2003 Jun 16. PubMed PMID: 12810728.
  9. Meyer-Wentrup F, Benitez-Ribas D, Tacken PJ, Punt CJ, Figdor CG, de Vries IJ, et al. Targeting DCIR on human plasmacytoid dendritic cells results in antigen presentation and inhibits IFN-alpha production. Blood. 2008 Apr 15;111(8):4245-53. doi: 10.1182/blood-2007-03-081398. Epub 2008 Feb 7. PubMed PMID: 18258799.
  10. Fromm JR. Flow cytometric analysis of CD123 is useful for immunophenotyping classical Hodgkin lymphoma. Cytometry B Clin Cytom. 2011 Mar;80(2):91-9. doi: 10.1002/cyto.b.20561. Epub 2010 Aug 31. PubMed PMID: 20809502.
  11. Lozza L, Farinacci M, Faé K, Bechtle M, Stäber M, Dorhoi A, et al. Crosstalk between human DC subsets promotes antibacterial activity and CD8+ T-cell stimulation in response to bacille Calmette-Guérin. Eur J Immunol. 2014 Jan;44(1):80-92. doi: 10.1002/eji.201343797. Epub 2013 Oct 20. PubMed PMID: 24114554. (Free PMC Article available, 8 images available)
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Alexa Fluor 488
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol
Specificity
Specificity:
human CD40
Gene ID 3383

Accessory Products

Proteins and/or Positive Controls

Proteins for CD40 (16 products)

Catalog No. Species Pres. Purity   Source  

CD40 (transcript variant 1)
CD40 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

CD40 (transcript variant 1)
CD40 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
  OriGene Technologies, Inc.

CD40 (21-193, His-tag)
CD40 Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €820.00
  OriGene Technologies GmbH

CD40 (21-193, His-tag)
CD40 Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €320.00
  OriGene Technologies GmbH

CD40
CD40 Human
  Abnova Taiwan Corp.

CD40
CD40 Human Human HEK293T cells
  Abnova Taiwan Corp.

CD40
CD40 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD40
CD40 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD40
CD40 Human Purified
  Abnova Taiwan Corp.

CD40
CD40 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD40
CD40 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD40
CD40 Human Purified
  Abnova Taiwan Corp.

CD40
CD40 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD40
CD40 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD40
Western Blot: CD40 Protein [NBP1-46044] - Lane 1 – MW markers; Lane 2 – CD40 (173aa) - FcB Chimera; Lane 3 – CD40 (173aa) - FcB Chimera treated with PNGase F to remove potential Nlinked glycans; Lane 4 – CD40 (173aa) - FcB Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie. Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. Human Purified
  Novus Biologicals Inc.

CD40
Western Blot: CD40 Protein [NBP1-46078] - Lane 1 – MW markers; Lane 2 – CD40 (167aa) - Fc Chimera; Lane 3 – CD40 (167aa) - Fc Chimera treated with PNGase F to remove potential N-linked glycans; Lane 4 – CD40 (167aa) - Fc Chimera treated with a glycosidase cocktail to remove potential N- and O-linked glycans. Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie. Drop in MW after treatment with PNGase F indicates the presence of N-linked glycans. Additional bands in lane 3 and lane 4 are glycosidase enzymes. Human Purified
  Novus Biologicals Inc.

Positive controls for CD40 (2 products)

Catalog No. Species Pres. Purity   Source  

CD40 293T Cell Transient Overexpression Lysate(Denatured)
CD40 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD40 Lysate
Western Blot: CD40 Lysate [NBL1-08948] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CD40
  Novus Biologicals Inc.

Recommended Secondary Antibodies for CD40 (11 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
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Click here to see all secondary antibodies for CD40
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