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DDX0192B-50 CD208 / LAMP3 antibody

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Rat anti Mouse CD208 / LAMP3 1006F7.05

DDX0192B-50

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  • DDX0192B-50

Product Description for CD208 / LAMP3

Rat anti Mouse CD208 / LAMP3 1006F7.05.
Presentation: Biotin
Product is tested for Flow Cytometry, Frozen Sections.

Properties for CD208 / LAMP3

Product Category Primary Antibodies
Quantity 50 µg
Synonyms DC-LAMP, DC-lysosome-associated membrane glycoprotein, DCLAMP, Dendritic Cell Marker, Lysosomal-associated membrane protein 3, Lysosome-associated membrane glycoprotein 3, Protein TSC403, TSC403
Presentation Biotin
Reactivity Ms
Applications C, F
Clonality Monoclonal
Clone 1006F7.05
Host Rat
Isotype IgG2a
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q7TST5
Immunogen:
recombinant murine DC-LAMP
GeneID:
239739
Application DDX0192P-50 / DDX0192P-100 Purified: Surface and Intra Flow cytometry, IHC, IF. 
DDX0192A488-50 / DDX0192A488-100 Alexa-fluor®488: Surface and Intra Flow cytometry, IF.
DDX0192A546-50 / DDX0192A546-100 Alexa- fluor®546: Surface and Intra Flow cytometry.
DDX0192A647-50 / DDX0192A647-100 Alexa- fluor®647: Surface and Intra Flow cytometry.
DDX0192B-50 / DDX0192B-100: Biotin (on request): Surface and Intra Flow cytometry, IHC. 
Usage recommendation:
*This monoclonal antibody may be used between 5-20μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in the mouse and 90 kDa in human (due to extensive glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. DC-LAMP is specifically expressed by human DCs upon activation. However, its mouse counterpart has not be detected in mature DCs. DC-LAMP is constitutively expressed by mouse. Confocal and immunoelectron microscopy have shown that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contains lung surfactant protein B.
(Salaun B et al, 2003 ; Eur. J. Immunol., 33 :9, 2619-29 ; Salaun B et al, 2004 ; Am. J. Pathol., 164 :3, 861-71 ; Rochereau N et al, Vaccine, 2011).
Concentration 0.5 mg/nml
Product Citations
Originator or purchased from resellers:
  1. de Saint-Vis B, Vincent J, Vandenabeele S, Vanbervliet B, Pin JJ, Aït-Yahia S, et al. A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment. Immunity. 1998 Sep;9(3):325-36. PubMed PMID: 9768752.
  2. Salaun B, de Saint-Vis B, Clair-Moninot V, Pin JJ, Barthélemy-Dubois C, Kissenpfennig A, et al. Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol. 2003 Sep;33(9):2619-29. PubMed PMID: 12938238.
  3. Salaun B, de Saint-Vis B, Pacheco N, Pacheco Y, Riesler A, Isaac S, et al. CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol. 2004 Mar;164(3):861-71. PubMed PMID: 14982840. (Free PMC Article available, 7 images available)
  4. Elliott B, Scolyer RA, Suciu S, Lebecque S, Rimoldi D, Gugerli O, et al. Long-term protective effect of mature DC-LAMP+ dendritic cell accumulation in sentinel lymph nodes containing micrometastatic melanoma. Clin Cancer Res. 2007 Jul 1;13(13):3825-30. PubMed PMID: 17606713.
  5. Rochereau N, Verrier B, Pin JJ, Genin C, Paul S. Phenotypic localization of distinct DC subsets in mouse Peyer Patch. Vaccine. 2011 May 9;29(20):3655-61. doi: 10.1016/j.vaccine.2011.03.012. Epub 2011 Mar 23. PubMed PMID: 21439318.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Biotin
Purification:
QMA hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol
Specificity
Specificity:
Murine DC-LAMP
Species cross-reactivity: none.
Gene ID 239739

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