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DDX0193A488-50 CD208 / LAMP3 antibody

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Mouse anti Human CD208 / LAMP3 109G3

DDX0193A488-50

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  • DDX0193A488-50

Product Description for CD208 / LAMP3

Mouse anti Human CD208 / LAMP3 109G3.
Presentation: Alexa Fluor 488
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.

Properties for CD208 / LAMP3

Product Category Primary Antibodies
Quantity 50 µg
Synonyms DC-LAMP, DC-lysosome-associated membrane glycoprotein, DCLAMP, Dendritic Cell Marker, Lysosomal-associated membrane protein 3, Lysosome-associated membrane glycoprotein 3, Protein TSC403, TSC403
Presentation Alexa Fluor 488
Reactivity Hu
Applications F, ICC/IF
Clonality Monoclonal
Clone 109G3
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UQV4
Immunogen:
Human DC-LAMP alkaline phosphatase fusion protein.
GeneID:
27074
Application DDX0193P-50 / DDX0193P-100 Purified: FACS intracellular, ImmunoHistoChemistry frozen sections. 
DDX0193A488-50 / DDX0193A488-100 Alexa-fluor®488: FACS intracellular, Immunofluorescence. 
DDX0193A546-50 / DDX0193A546-100 Alexa-fluor®546: Immunofluorescence. 
DDX0193A647-50 / DDX0193A647-100 Alexa-fluor®647: FACS intracellular. 
DDX0193B-50 / DDX0193B-100 Biotin (on request): FACS intracellular, ImmunoHistoChemistry frozen sections.
Background The dendritic cell lysosomal-associated membrane protein (DC-LAMP)/CD208 is a type I integral transmembrane glycoprotein mostly homologous to CD68, of about 45 kDa in the mouse and 90 kDa in human (due to extensive glycosylation), with a bipartite C-terminal structure divided by a serine/proline rich region, a transmembrane domain and a conserved tyrosine-based lysosomal targeting motif in its cytoplasmic tail. Initially cloned as a specific marker of human mature dendritic cells (DCs), DC-LAMP has been subsequently shown to be expressed in alveolar type II pneumocytes. In both cell types, the molecule is found in the limiting membrane of intracellular multi-lamellar bodies, known as MIIC (MHC class II compartments) in human mature DC and as lung surfactant-containing lamellar bodies in type II pneumocytes. In the latter cell type, DC-LAMP expression is also detected at the cell surface.
(de Saint-Vis B et al, 1998 ; Immunity ; 9: 325-336)
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. de Saint-Vis B, Vincent J, Vandenabeele S, Vanbervliet B, Pin JJ, Aït-Yahia S, et al. A novel lysosome-associated membrane glycoprotein, DC-LAMP, induced upon DC maturation, is transiently expressed in MHC class II compartment. Immunity. 1998 Sep;9(3):325-36. PubMed PMID: 9768752.
  2. Elliott B, Scolyer RA, Suciu S, Lebecque S, Rimoldi D, Gugerli O, et al. Long-term protective effect of mature DC-LAMP+ dendritic cell accumulation in sentinel lymph nodes containing micrometastatic melanoma. Clin Cancer Res. 2007 Jul 1;13(13):3825-30. PubMed PMID: 17606713.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Alexa Fluor 488
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol
Specificity
Specificity:
Human DC-LAMP (epitope in intracytoplasmic domain).
Gene ID 27074

Accessory Products

Proteins and/or Positive Controls

Proteins for CD208 / LAMP3 (4 products)

Catalog No. Species Pres. Purity   Source  

CD208 / LAMP3 (28-381, His-tag)

CD208 / LAMP3 Human Purified > 90 % by SDS – PAGE Insect cells
0.25 mg / €1,070.00
  OriGene Technologies GmbH

CD208 / LAMP3 (28-381, His-tag)

CD208 / LAMP3 Human Purified > 90 % by SDS – PAGE Insect cells
50 µg / €320.00
  OriGene Technologies GmbH

CD208 / LAMP3

CD208 / LAMP3 Human Human HEK293T cells
  Abnova Taiwan Corp.

CD208 / LAMP3

CD208 / LAMP3 Human
  Abnova Taiwan Corp.

Positive controls for CD208 / LAMP3 (2 products)

Catalog No. Species Pres. Purity   Source  

LAMP3 Lysate

Western Blot: LAMP3 Lysate [NBL1-12432] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for LAMP3
  Novus Biologicals Inc.

LAMP3 overexpression lysate

LAMP3 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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