Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » CD209 / DC-SIGN
discontinued

DDX0202P-50 CD209 / DC-SIGN antibody

See related secondary antibodies

Search for all "CD209 / DC-SIGN"

View Datasheet

Quick Overview

Mouse anti Canine, Human CD209 / DC-SIGN 102E11.06

DDX0202P-50

More Views

  • DDX0202P-50
  • DDX0202P-50

Product Description for CD209 / DC-SIGN

Mouse anti Canine, Human CD209 / DC-SIGN 102E11.06.
Presentation: Purified
Product is tested for Frozen Sections, Paraffin Sections, Flow Cytometry.

Properties for CD209 / DC-SIGN

Product Category Primary Antibodies
Quantity 50 µg
Synonyms C-type lectin domain family 4 member L, CLEC4L, DC-SIGN1, DCSIGN, DCSIGN1, Dendritic Cell Marker, Dendritic cell-specific ICAM-3-grabbing non-integrin 1
Presentation Purified
Reactivity Can, Hu
Applications C, F, P
Clonality Monoclonal
Clone 102E11.06
Host Mouse
Isotype IgG2b
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9NNX6
Immunogen:
HeLa cells stably transfected-with human DC-SIGN.
GeneID:
30835
Isotype control AM03110PU-N, SM12P
Application DDX0202P-50 / DDX0202P-100 Purified: FACS surface, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections (formol). 
DDX0202A488-50 / DDX0202A488-100 Alexa-fluor®488: Surface Flow cytometry, Immunofluorescence.
DDX0202A546-50 / DDX0202A546-100 Alexa- fluor®546: Immunofluorescence.
DDX0202A647-50 / DDX0202A647-100 Alexa- fluor®647: Surface Flow cytometry
DDX0202B-50 / DDX0202B-100 Biotin (on request): FACS surface, ImmunoHistoChemistry frozen sections, ImmunoHistoChemistry paraffin sections. 
Usage recommendation:
*This monoclonal antibody may be used between 1-20 μg/ml.
Background DC-SIGN (“DC Specific, ICAM-3 Grabbing, Nonintegrin”) / CD209 and liver/lymph node-specific ICAM- 3-grabbing nonintegrin (L-SIGN) (CD299/DC-SIGNR for DC-SIGN-related molecule; DC-SIGN2) are closely related genes that map to chromosome 19p13.3.Both genes encode a member of the C-type lectin family of type II transmembrane proteins. The two receptors are 77% identical at the amino acid level, have similar ligands. They are expressed in different tissues. DC-SIGN is expressed on dendritic cells and macrophages. L-SIGN is found in the endothelial cells of liver, lymph nodes, and placenta and is absent on DCs and macrophages. Both receptors have been shown to interact with ICAM-3 DC-SIGN is a high affinity receptor for HIV gp120, (Soilleux EJ. 2003, Clinical Science 104, 437; Dakappagari N., et al. 2006, The Journal of Immunology,176, 426-; Geijtenbeeck T.B., et al. 2000, Cell, 100, 575). Bashirova A. et al., 2001, J.Exp. Med., 193, 671).Antibodies have been selected with NIH3T3 transfected cells with either LSIGN or DC-SIGN.
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Bashirova AA, Geijtenbeek TB, van Duijnhoven GC, van Vliet SJ, Eilering JB, Martin MP, et al. A dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein is highly expressed on human liver sinusoidal endothelial cells and promotes HIV-1 infection. J Exp Med. 2001 Mar 19;193(6):671-8. PubMed PMID: 11257134. (Free PMC Article available, 5 images available)
  2. Soilleux EJ. DC-SIGN (dendritic cell-specific ICAM-grabbing non-integrin) and DC-SIGN-related (DC-SIGNR): friend or foe? Clin Sci (Lond). 2003 Apr;104(4):437-46. PubMed PMID: 12653690.
  3. Dakappagari N, Maruyama T, Renshaw M, Tacken P, Figdor C, Torensma R, et al. Internalizing antibodies to the C-type lectins, L-SIGN and DC-SIGN, inhibit viral glycoprotein binding and deliver antigen to human dendritic cells for the induction of T cell responses. J Immunol. 2006 Jan 1;176(1):426-40. PubMed PMID: 16365436.
  4. Rochereau N, Verrier B, Pin JJ, Genin C, Paul S. Phenotypic localization of distinct DC subsets in mouse Peyer Patch. Vaccine. 2011 May 9;29(20):3655-61. doi: 10.1016/j.vaccine.2011.03.012. Epub 2011 Mar 23. PubMed PMID: 21439318.
  5. Debeer S, Le Luduec JB, Kaiserlian D, Laurent P, Nicolas JF, Dubois B, et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol. 2013 Jul-Aug;23(4):456-66. doi: 10.1684/ejd.2013.2060. PubMed PMID: 24047577.
  6. Berbic M, Ng CH, Black K, Markham R, Russell P, Basten A, et al. A novel pilot study of endometrial stromal cells and immune cell populations in sentinel uterine-draining lymph nodes during the menstrual cycle and in endometriosis. Reprod Sci. 2013 Nov;20(11):1339-48. doi: 10.1177/1933719113485298. Epub 2013 May 29. PubMed PMID: 23719712.
General Readings
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
State:
Purified
Specificity
Specificity:
human L-SIGN and human DC-SIGN. 
Species cross-reactivity: human L-SIGN , dog.
Gene ID 30835

Accessory Products

Proteins and/or Positive Controls

Proteins for CD209 / DC-SIGN (6 products)

Catalog No. Species Pres. Purity   Source  

CD209 / DC-SIGN
CD209 / DC-SIGN Human
  Abnova Taiwan Corp.

CD209 / DC-SIGN
CD209 / DC-SIGN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD209 / DC-SIGN
CD209 / DC-SIGN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD209 / DC-SIGN
CD209 / DC-SIGN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD209 / DC-SIGN
CD209 / DC-SIGN Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD209 / DC-SIGN
Densitometry: DC-SIGN Protein [NBP1-46043] - Post-translational modifications result in protein heterogeneity. The densitometry scan, derived from the 2D gel image above, demonstrates the purified human cell expressed protein exists in multiple glycoforms, which differ according to their level of post-translational modification. Expression of these glycoforms is highly significant for cell biology, as they more closely resemble the native human proteins. The triangle indicates theoretical pI and MW. Human Purified
  Novus Biologicals Inc.

Positive controls for CD209 / DC-SIGN (2 products)

Catalog No. Species Pres. Purity   Source  

CD209 293T Cell Transient Overexpression Lysate(Denatured)
CD209 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD209 / DC-SIGN Lysate
Western Blot: DC-SIGN Lysate [NBL1-08921] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CD209
  Novus Biologicals Inc.

Recommended Secondary Antibodies for CD209 / DC-SIGN (11 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
    OriGene Technologies GmbH
Click here to see all secondary antibodies for CD209 / DC-SIGN
Compare Products

You have no items to compare.

My Cart

You have no items in your shopping cart.

Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn