discontinued

DDX0220A488-50 DORA / IGSF6 antibody

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Mouse anti Canine, Human, Mouse DORA / IGSF6 104A10.01

DDX0220A488-50

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  • DDX0220A488-50
  • DDX0220A488-50

Product Description for DORA / IGSF6

Mouse anti Canine, Human, Mouse DORA / IGSF6 104A10.01.
Presentation: Alexa Fluor 488
Product is tested for Western blot / Immunoblot, Flow Cytometry.

Properties for DORA / IGSF6

Product Category Primary Antibodies
Quantity 50 µg
Synonyms Immunoglobulin superfamily member 6
Presentation Alexa Fluor 488
Reactivity Can, Hu, Ms
Applications F, WB
Clonality Monoclonal
Clone 104A10.01
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
O95976
Immunogen:
Human recombinant DORA Ig fusion protein.
GeneID:
10261
Application Flow Cytometry surface, Western Blot.
Usage recommendation:

*This monoclonal antibody may be used between 5-15 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background Using a cDNA subtraction technique, a novel member of the immunoglobulin superfamily was isolated from human dendritic cells (DCs). This cDNA, named DORA, for Down-Regulated by Activation encodes a protein belonging to the CD8 family of receptors containing a single V type loop domain with an associated J chain region, a transmembrane region containing an atypical tyrosine residue and a cytoplasmic domain containing three putative tyrosine phosphorylation sites. The human DORA gene has been mapped to chromosome 16. Expression is observed in DCs, purified ex vivo or generated in vitro from either monocytes or CD34+ progenitors, and down-regulated following activation by PMA and Ionomycin or by CD40L engagement.
(Bates EE et al, 2000; Immunogenetics, 52: 112-20; Bates EE et al, 1998; Mol. Immunology, 35: 513-24).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Rochereau N, Verrier B, Pin JJ, Genin C, Paul S. Phenotypic localization of distinct DC subsets in mouse Peyer Patch. Vaccine. 2011 May 9;29(20):3655-61. doi: 10.1016/j.vaccine.2011.03.012. Epub 2011 Mar 23. PubMed PMID: 21439318.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Alexa Fluor 488
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
Specificity
Specificity:
Human DORA. 
Species cross-reactivity: Mouse, Canine.
Gene ID 10261

Accessory Products

Proteins and/or Positive Controls

Proteins for DORA / IGSF6 (3 products)

Catalog No. Species Pres. Purity   Source  

DORA / IGSF6

DORA / IGSF6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

DORA / IGSF6

DORA / IGSF6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

DORA / IGSF6

DORA / IGSF6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for DORA / IGSF6 (3 products)

Catalog No. Species Pres. Purity   Source  

IGSF6 293T Cell Transient Overexpression Lysate(Denatured)

IGSF6 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

IGSF6 overexpression lysate

IGSF6 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

Immunoglobulin superfamily member 6 Lysate

Western Blot: DORA Lysate [NBL1-11884] - Western Blot experiments. 
Left-Control; Right -Over-expression Lysate for IGSF6
  Novus Biologicals Inc.
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