discontinued

DDX0230P-50 PILRA antibody

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Rat anti Human PILRA 36H2

DDX0230P-50

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  • DDX0230P-50
  • DDX0230P-50

Product Description for PILRA

Rat anti Human PILRA 36H2.
Presentation: Purified
Product is tested for Immunoprecipitation, Western blot / Immunoblot, Flow Cytometry.

Properties for PILRA

Product Category Primary Antibodies
Quantity 50 µg
Synonyms Cell surface receptor FDF03, FDF03, Inhibitory receptor PILR-alpha, PILR-alpha, Paired immunoglobulin-like type 2 receptor alpha
Presentation Purified
Reactivity Hu
Applications F, IP, WB
Clonality Monoclonal
Clone 36H2
Host Rat
Isotype IgG2a
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UKJ1
Immunogen:
Human recombinant FDF03-Ig fusion protein.
GeneID:
29992
Isotype control SM15P, SM15PX
Application DDX0230P-50 / DDX0230P-100 Purified: Surface Flow cytometry,  Immunoprecipitation, Western Blot.
DDX0230A488-50 / DDX0230A488-100 Alexa-fluor®488: Surface Flow cytometry, ImmunoFluorescence.
DDX0230A546-50 / DDX0230A546-100 Alexa-fluor®488: Surface Flow cytometry, ImmunoFluorescence. 
DDX0230A647-50 / DDX0230A647-100 Alexa-fluor®488: Surface Flow cytometry, ImmunoFluorescence. 
DDX0230B-50 / DDX0230B-100 Biotin: Western Blot.

Usage recommendation:

*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background Human FDF03, is a novel member of the Ig superfamily expressed as a monomeric 44 kDa transmembrane glycoprotein and containing a single extracellular V-set Ig-like domain. Two potential secreted isoforms were also identified. FDF03 was mostly detected in hemopoietic tissues and was expressed by monocytes, macrophages, and granulocytes, but not by lymphocytes (B, T, and NK cells), indicating an expression restricted to the myelomonocytic lineage. FDF03 was also strongly expressed by monocyte-derived dendritic cells (moDC) and preferentially by CD14+/CD1a- DC derived from CD34+ progenitors. Moreover, flow cytometric analysis showed FDF03 expression by CD11c+ blood and tonsil DC, but not by CD11c- DC precursors. The FDF03 cytoplasmic tail contained two immunoreceptor tyrosine-based inhibitory motifs (ITIM)-like sequences. Human FDF03 is a novel ITIM-bearing receptor selectively expressed by cells of myeloid origin, including DC, which may regulate functions other than that of the broadly distributed LAIR-1/p40 molecule. (Fournier N et al, 2000; J. Immunol., 165(3):1197-1209).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Fournier N, Chalus L, Durand I, Garcia E, Pin JJ, Churakova T, et al. FDF03, a novel inhibitory receptor of the immunoglobulin superfamily, is expressed by human dendritic and myeloid cells. J Immunol. 2000 Aug 1;165(3):1197-209. PubMed PMID: 10903717.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200 μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200 μl / 50 μg in 100 μl PBS 50% glycerol.
State:
Purified
Specificity
Specificity:
Human FDF03.
Gene ID 29992

Accessory Products

Proteins and/or Positive Controls

Proteins for PILRA (2 products)

Catalog No. Species Pres. Purity   Source  

PILRA

PILRA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

PILRA

PILRA Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for PILRA (2 products)

Catalog No. Species Pres. Purity   Source  

PILRA overexpression lysate

PILRA overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

PILRA overexpression lysate

PILRA overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.
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