Product Description for IRAK4
Mouse anti Canine, Human IRAK4 6F8.
Presentation: Alexa Fluor 488
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.
Presentation: Alexa Fluor 488
Product is tested for Immunocytochemistry/Immunofluorescence, Flow Cytometry.
Properties for IRAK4
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 50 µg |
| Synonyms | IRAK-4, Interleukin-1 receptor-associated kinase-like, NY-REN-64 |
| Presentation | Alexa Fluor 488 |
| Reactivity | Can, Hu |
| Applications | F, ICC/IF |
| Clonality | Monoclonal |
| Clone | 6F8 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Germany, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Dendritics SAS |
Datasheet Extract
| Immunogen |
|
|---|---|
| Application | DDX0340P-50 / DDX0340P-100 Purified: IHC paraffin, IF, Intracellular flow cytometry, WB. DDX0340A488-50 / DDX0340A488-100 Alexa-fluor®488: IF, Intracellular flow cytometry. DDX0340A546-50 / DDX0340A546-100 Alexa-fluor®546: IF, Intracellular flow cytometry. DDX0340A647-50 / DDX0340A647-100 Alexa-fluor®647: IF, Intracellular flow cytometry. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
| Background | IL-1R associated kinases (IRAKs) are important mediators in the signal transduction of Toll / IL-1R (TIR) family members. The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK-M and IRAK4). IL-1 stimulation leads to the recruitment of the IL-1R associated kinase (IRAK) to the IL-1 Receptor where IRAK is phosphorylated, ubiquinated, and eventually degraded. Endogenous IRAK-4 interacts with IRAK-1 and TRAF6 in an IL-1 dependent manner. IRAK-4 is able to phosphorylate IRAK-1. The 6F8 clone was generated after mouse immunization with human IRAK4-transfected eukaryotic cells. This monoclonal antibody was demonstrated to cross-react with canine IRAK-4. (Cao S. et al, 1996 ; Science, 271, 1128-1131 ; Li S. et al, 2002 ; PNAS, 99, 5567-5572, Bonnefont C, personal communication, 2011). |
| Concentration | 0.5 mg/ml |
| General Readings | DDX-Veterinary tools |
| Storage | -20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing. |
| Format |
|
| Specificity |
|
| Gene ID | 51135 |
Accessory Products
Proteins and/or Positive Controls
Proteins for IRAK4 (7 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP303041 |
IRAK4 (transcript variant 2) |
||||||
|
Human |
> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€748.00
|
|||
| OriGene Technologies, Inc. | |||||||
| H00051135-P01-10 |
IRAK4 |
||||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00051135-P01-25 |
IRAK4 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00051135-Q01-10 |
IRAK4 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00051135-Q01-25 |
IRAK4 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P2809 |
IRAK4 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| P5566 |
IRAK4 |
||||||
|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
Positive controls for IRAK4 (2 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| L027T6 |
IRAK4 HEK293 Cell Transient Overexpression Lysate (Non-Denatured) |
||||||
|
Transient overexpression cell lysate was tested with Anti-IRAK4 antibody () by Western Blots. | ||||||
| Abnova Taiwan Corp. | |||||||
| NBL1-12028 |
IRAK4 Lysate |
||||||
![Western Blot: IRAK4 Lysate [NBL1-12028] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for IRAK4](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/IRAK4%20Lysate-Western%20Blot-NBL1-12028-img0001.jpg) |
|||||||
| Novus Biologicals Inc. | |||||||
Recommended Secondary Antibodies for IRAK4 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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