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Home » CD207 / Langerin
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DDX0360P-50 CD207 / Langerin antibody

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Highly validated antibody!

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Mouse anti Human, Mouse CD207 / Langerin 808E10.01

DDX0360P-50

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  • DDX0360P-50
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Product Description for CD207 / Langerin

Mouse anti Human, Mouse CD207 / Langerin 808E10.01.
Presentation: Purified
Product is tested for Frozen Sections, Functional assay, Flow Cytometry.

Properties for CD207 / Langerin

Product Category Primary Antibodies
Quantity 50 µg
Synonyms C-type lectin domain family 4 member K, CLEC4K, Dendritic Cell Marker
Presentation Purified
Reactivity Hu, Ms
Applications C, F, FN
Clonality Monoclonal
Clone 808E10.01
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9UJ71
Immunogen:
Human Langerin-transfected eukaryotic cells
GeneID:
50489
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Flow cytometry, blocks HIVgp120, IHC. 

Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Background Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system.
(Valladeau J et al, 2002; J. Immunol., 168 : 782-779 ; Douillard P et al, 200; J. Invest. Dermatol., 125: 983–994)
Concentration 0.5 mg/ml
Protocols Langerin-gp120 blocking protocol:
Mock- and Langerin-transfected CHO cells are incubated with biotin-labelled gp160 (5μg/ml) and clone 808E10.01 at (1 to 10μg/ml) in PBS 45 min. at RT. Cells were washed in PBS 5min at 1600rpm, and stained with Streptavidin-PE 30 min at 4°C (DAKO). A last PBS wash is performed before Flow cytometry analysis.
Product Citations
Originator or purchased from resellers:
  1. Valladeau J, Clair-Moninot V, Dezutter-Dambuyant C, Pin JJ, Kissenpfennig A, Mattéi MG, et al. Identification of mouse langerin/CD207 in Langerhans cells and some dendritic cells of lymphoid tissues. J Immunol. 2002 Jan 15;168(2):782-92. PubMed PMID: 11777972.
  2. Douillard P, Stoitzner P, Tripp CH, Clair-Moninot V, Aït-Yahia S, McLellan AD, et al. Mouse lymphoid tissue contains distinct subsets of langerin/CD207 dendritic cells, only one of which represents epidermal-derived Langerhans cells. J Invest Dermatol. 2005 Nov;125(5):983-94. PubMed PMID: 16297200.
  3. Nfon CK, Dawson H, Toka FN, Golde WT. Langerhans cells in porcine skin. Vet Immunol Immunopathol. 2008 Dec 15;126(3-4):236-47. doi: 10.1016/j.vetimm.2008.07.012. Epub 2008 Aug 3. PubMed PMID: 18805593.
  4. Bobr A, Olvera-Gomez I, Igyarto BZ, Haley KM, Hogquist KA, Kaplan DH. Acute ablation of Langerhans cells enhances skin immune responses. J Immunol. 2010 Oct 15;185(8):4724-8. doi: 10.4049/jimmunol.1001802. Epub 2010 Sep 20. PubMed PMID: 20855870. (Free PMC Article available, 4 images available)
  5. de Jong MA, de Witte L, Taylor ME, Geijtenbeek TB. Herpes simplex virus type 2 enhances HIV-1 susceptibility by affecting Langerhans cell function. J Immunol. 2010 Aug 1;185(3):1633-41. doi: 10.4049/jimmunol.0904137. Epub 2010 Jun 30. PubMed PMID: 20592277.
  6. Rochereau N, Verrier B, Pin JJ, Genin C, Paul S. Phenotypic localization of distinct DC subsets in mouse Peyer Patch. Vaccine. 2011 May 9;29(20):3655-61. doi: 10.1016/j.vaccine.2011.03.012. Epub 2011 Mar 23. PubMed PMID: 21439318.
  7. Schwingshackl P, Obermoser G, Nguyen VA, Fritsch P, Sepp N, Romani N. Distribution and maturation of skin dendritic cell subsets in two forms of cutaneous T-cell lymphoma: mycosis fungoides and Sézary syndrome. Acta Derm Venereol. 2012 May;92(3):269-75. doi: 10.2340/00015555-1220. PubMed PMID: 22678564.
  8. Luckashenak N, Wähe A, Breit K, Brakebusch C, Brocker T. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol. 2013 Jan 1;190(1):27-35. doi: 10.4049/jimmunol.1201082. Epub 2012 Dec 3. PubMed PMID: 23209325.
  9. Iram N, Mildner M, Prior M, Petzelbauer P, Fiala C, Hacker S, et al. Age-related changes in expression and function of Toll-like receptors in human skin. Development. 2012 Nov;139(22):4210-9. doi: 10.1242/dev.083477. Epub 2012 Oct 3. PubMed PMID: 23034637. (Free PMC Article available, 4 images available)
  10. Hitzler M, Majdic O, Heine G, Worm M, Ebert G, Luch A, et al. Human Langerhans cells control Th cells via programmed death-ligand 1 in response to bacterial stimuli and nickel-induced contact allergy. PLoS One. 2012;7(10):e46776. doi: 10.1371/journal.pone.0046776. Epub 2012 Oct 9. PubMed PMID: 23056446. (Free PMC Article available, 6 images available)
  11. Segura E, Valladeau-Guilemond J, Donnadieu MH, Sastre-Garau X, Soumelis V, Amigorena S. Characterization of resident and migratory dendritic cells in human lymph nodes. J Exp Med. 2012 Apr 9;209(4):653-60. doi: 10.1084/jem.20111457. Epub 2012 Mar 19. PubMed PMID: 22430490. (Free PMC Article available, 7 images available)
  12. Debeer S, Le Luduec JB, Kaiserlian D, Laurent P, Nicolas JF, Dubois B, et al. Comparative histology and immunohistochemistry of porcine versus human skin. Eur J Dermatol. 2013 Jul-Aug;23(4):456-66. doi: 10.1684/ejd.2013.2060. PubMed PMID: 24047577.
  13. De Jesus M, Ostroff GR, Levitz SM, Bartling TR, Mantis NJ. A population of Langerin-positive dendritic cells in murine Peyer's patches involved in sampling β-glucan microparticles. PLoS One. 2014 Mar 14;9(3):e91002. doi: 10.1371/journal.pone.0091002. eCollection 2014. PubMed PMID: 24632738. (Free PMC Article available, 6 images available)
General Readings
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Tris-NaCl pH 8
State:
Purified
Specificity
Specificity:
Human Langerin (extracellular epitope). 
Species cross-reactivity: Mouse.
Gene ID 50489

Accessory Products

Proteins and/or Positive Controls

Proteins for CD207 / Langerin (8 products)

Catalog No. Species Pres. Purity   Source  

CD207 / Langerin
CD207 / Langerin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD207 / Langerin (65-328, His-tag)
CD207 / Langerin Human Purified > 85 % by SDS - PAGE E. coli
0.5 mg / €820.00
  OriGene Technologies GmbH

CD207 / Langerin (65-328, His-tag)
CD207 / Langerin Human Purified > 85 % by SDS - PAGE E. coli
0.1 mg / €320.00
  OriGene Technologies GmbH

CD207 / Langerin (65-328, His-tag)
CD207 / Langerin Human Purified > 90 % by SDS - PAGE
0.25 mg / €1,070.00
  OriGene Technologies GmbH

CD207 / Langerin (65-328, His-tag)
CD207 / Langerin Human Purified > 90 % by SDS - PAGE
50 µg / €320.00
  OriGene Technologies GmbH

CD207 / Langerin
CD207 / Langerin Human
  Abnova Taiwan Corp.

CD207 / Langerin
CD207 / Langerin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

CD207 / Langerin
CD207 / Langerin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for CD207 / Langerin (4 products)

Catalog No. Species Pres. Purity   Source  

CD207 293T Cell Transient Overexpression Lysate(Denatured)
CD207 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

CD207 / Langerin Lysate(Denatured)
CD207 / Langerin Lysate(Denatured)
  Abnova Taiwan Corp.

CD207 overexpression lysate
CD207 overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

Langerin Lysate
Western Blot: Langerin Lysate [NBL1-08920] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for CD207
  Novus Biologicals Inc.

Recommended Secondary Antibodies for CD207 / Langerin (11 products)

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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
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Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
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Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
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Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
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