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DDX0390P-50 CD317 / BST2 (Plasmacytoid dendritic cells / pDCs) antibody

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Rat anti Mouse CD317 / BST2 120G8.04

DDX0390P-50

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Product Description for CD317 / BST2

Rat anti Mouse CD317 / BST2 120G8.04.
Properties: (Plasmacytoid dendritic cells / pDCs)
Presentation: Purified
Product is tested for Frozen Sections, Functional assay, Flow Cytometry.

Properties for CD317 / BST2

Product Category Primary Antibodies
Quantity 50 µg
Synonyms BST-2, Bone marrow stromal antigen 2, HM1.24 antigen, Tetherin
Presentation Purified
Reactivity Ms
Applications C, F, FN
Clonality Monoclonal
Clone 120G8.04
Host Rat
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q8R2Q8
Immunogen:
Mouse plasmatocytoid DCs (pDCs)
GeneID:
69550
Property Plasmacytoid dendritic cells / pDCs
Isotype control SM25P, SM25PX
Application DDX0390P-50 / DDX0390P-100 Purified: FACS surface, ImmunoHistoChemistry frozen sections, In vivo depletion.  
DDX0390A488-50 / DDX0390A488-100 Alexa-fluor®488: FACS surface, ImmunoFluorescence..
DDX0390A546-50 / DDX0390A546-100 Alexa-fluor®546: ImmunoFluorescence. 
DDX0390A647-50 / DDX0390A647-100 Alexa-fluor®647: Flow Cytometry. 
DDX0390B-50 / DDX0390B-100 Biotin: FACS surface, ImmunoHistoChemistry frozen sections.
DDX0390-HD01 1 mg Purified: In vivo Depletion. 
DDX0390-HD05 5 mg Purified:  In vivo Depletion. 
DDX0390-HD10 10 mg Purified: In vivo Depletion. 

Usage recommendation:
This monoclonal antibody may be used between at 1-10 μg/ml.
For pDCs in vivo depletion in Balb /c mice, mAb 120G8 was used between 50-200 μg / injection.
Optimal dilution should be determined by each laboratory for each application.
Background We generated rat monoclonal antibody (mAb) that recognizes mouse plasmacytoid dendritic cells (pDCs). The target molecule was found to be BST2 (bone marrow stromal cell antigen 2). This antibody, named 120G8, stains a small subset of CD11clow spleen cells with high specificity. This population produces high amounts of IFNα upon in vitro viral stimulation. Both ex vivo- and in vitro-derived 120G8+ cells display a phenotype identical with that of mouse pDCs (B220highLy6ChighGr1lowCD11b-CD11clow). Mice treated with 120G8 mAb are depleted of B220highLy6ChighCD11clow cells and have a much reduced ability to produce IFN in response to in vivo CpG stimulation. mAb 120G8 stains all and only B220highLy6ChighCD11clow pDC in all lymphoid organs. Immunohistochemical studies performed with this mAb indicate that pDC are located in the T cell area of spleen, lymph nodes, and Peyer's patches. Using 120G8 mAb in immunofluorescence studies demonstrates mouse strain- and organ-specific differences in the frequency of pDCs and other DC subsets (Asselin-Paturel C et al, 2003 ; J. Immunol., 172:6466; Blasius AI, 2006, J. Immunol., 177:3260 ; Goubier A et Al, 2008, Immunity, 29:464-475).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Asselin-Paturel C, Brizard G, Pin JJ, Brière F, Trinchieri G. Mouse strain differences in plasmacytoid dendritic cell frequency and function revealed by a novel monoclonal antibody. J Immunol. 2003 Dec 15;171(12):6466-77. PubMed PMID: 14662846.
  2. Fallarino F, Asselin-Paturel C, Vacca C, Bianchi R, Gizzi S, Fioretti MC, et al. Murine plasmacytoid dendritic cells initiate the immunosuppressive pathway of tryptophan catabolism in response to CD200 receptor engagement. J Immunol. 2004 Sep 15;173(6):3748-54. PubMed PMID: 15356121.
  3. Budimir N, de Haan A, Meijerhof T, Waijer S, Boon L, Gostick E, et al. Critical role of TLR7 signaling in the priming of cross-protective cytotoxic T lymphocyte responses by a whole inactivated influenza virus vaccine. PLoS One. 2013 May 2;8(5):e63163. doi: 10.1371/journal.pone.0063163. Print 2013. PubMed PMID: 23658804. (Free PMC Article available, 10 images available)
  4. Mellor AL, Baban B, Chandler PR, Manlapat A, Kahler DJ, Munn DH. Cutting edge: CpG oligonucleotides induce splenic CD19+ dendritic cells to acquire potent indoleamine 2,3-dioxygenase-dependent T cell regulatory functions via IFN Type 1 signaling. J Immunol. 2005 Nov 1;175(9):5601-5. PubMed PMID: 16237046.
  5. Blasius AL, Giurisato E, Cella M, Schreiber RD, Shaw AS, Colonna M. Bone marrow stromal cell antigen 2 is a specific marker of type I IFN-producing cells in the naive mouse, but a promiscuous cell surface antigen following IFN stimulation. J Immunol. 2006 Sep 1;177(5):3260-5. PubMed PMID: 16920966.
  6. Massberg S, Schaerli P, Knezevic-Maramica I, Köllnberger M, Tubo N, Moseman EA, et al. Immunosurveillance by hematopoietic progenitor cells trafficking through blood, lymph, and peripheral tissues. Cell. 2007 Nov 30;131(5):994-1008. PubMed PMID: 18045540. (Free PMC Article available, 7 images available)
  7. Wendland M, Czeloth N, Mach N, Malissen B, Kremmer E, Pabst O, et al. CCR9 is a homing receptor for plasmacytoid dendritic cells to the small intestine. Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6347-52. Epub 2007 Apr 2. PubMed PMID: 17404233. (Free PMC Article available, 5 images available)
  8. GeurtsvanKessel CH, Willart MA, van Rijt LS, Muskens F, Kool M, Baas C, et al. Clearance of influenza virus from the lung depends on migratory langerin+CD11b- but not plasmacytoid dendritic cells. J Exp Med. 2008 Jul 7;205(7):1621-34. doi: 10.1084/jem.20071365. PubMed PMID: 18591406. (Free PMC Article available, 7 images available)
  9. Goubier A, Dubois B, Gheit H, Joubert G, Villard-Truc F, Asselin-Paturel C, et al. Plasmacytoid dendritic cells mediate oral tolerance. Immunity. 2008 Sep 19;29(3):464-75. doi: 10.1016/j.immuni.2008.06.017. PubMed PMID: 18789731. (Free PMC Article available, 7 images available)
  10. Mascarell L, Lombardi V, Louise A, Saint-Lu N, Chabre H, Moussu H, et al. Oral dendritic cells mediate antigen-specific tolerance by stimulating TH1 and regulatory CD4+ T cells. J Allergy Clin Immunol. 2008 Sep;122(3):603-9.e5. doi: 10.1016/j.jaci.2008.06.034. PubMed PMID: 18774396.
  11. Jongbloed SL, Benson RA, Nickdel MB, Garside P, McInnes IB, Brewer JM. Plasmacytoid dendritic cells regulate breach of self-tolerance in autoimmune arthritis. J Immunol. 2009 Jan 15;182(2):963-8. PubMed PMID: 19124739.
  12. Seeds RE, Gordon S, Miller JL. Characterisation of myeloid receptor expression and interferon alpha/beta production in murine plasmacytoid dendritic cells by flow cytomtery. J Immunol Methods. 2009 Oct 31;350(1-2):106-17. doi: 10.1016/j.jim.2009.07.016. Epub 2009 Aug 7. PubMed PMID: 19666024.
  13. Mascarell L, Lombardi V, Zimmer A, Louise A, Tourdot S, Van Overtvelt L, et al. Mapping of the lingual immune system reveals the presence of both regulatory and effector CD4+ T cells. Clin Exp Allergy. 2009 Dec;39(12):1910-9. doi: 10.1111/j.1365-2222.2009.03337.x. Epub 2009 Aug 20. PubMed PMID: 19694757.
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  15. Diana J, Brezar V, Beaudoin L, Dalod M, Mellor A, Tafuri A, et al. Viral infection prevents diabetes by inducing regulatory T cells through NKT cell-plasmacytoid dendritic cell interplay. J Exp Med. 2011 Apr 11;208(4):729-45. doi: 10.1084/jem.20101692. Epub 2011 Mar 28. PubMed PMID: 21444661. (Free PMC Article available, 7 images available)
  16. Rochereau N, Verrier B, Pin JJ, Genin C, Paul S. Phenotypic localization of distinct DC subsets in mouse Peyer Patch. Vaccine. 2011 May 9;29(20):3655-61. doi: 10.1016/j.vaccine.2011.03.012. Epub 2011 Mar 23. PubMed PMID: 21439318.
  17. Liu X, Mishra P, Yu S, Beckmann J, Wendland M, Kocks J, et al. Tolerance induction towards cardiac allografts under costimulation blockade is impaired in CCR7-deficient animals but can be restored by adoptive transfer of syngeneic plasmacytoid dendritic cells. Eur J Immunol. 2011 Mar;41(3):611-23. doi: 10.1002/eji.201040877. Epub 2011 Feb 10. PubMed PMID: 21341262.
  18. Seth S, Oberdörfer L, Hyde R, Hoff K, Thies V, Worbs T, et al. CCR7 essentially contributes to the homing of plasmacytoid dendritic cells to lymph nodes under steady-state as well as inflammatory conditions. J Immunol. 2011 Mar 15;186(6):3364-72. doi: 10.4049/jimmunol.1002598. Epub 2011 Feb 4. PubMed PMID: 21296980.
  19. Soloff AC, Weirback HK, Ross TM, Barratt-Boyes SM. Plasmacytoid dendritic cell depletion leads to an enhanced mononuclear phagocyte response in lungs of mice with lethal influenza virus infection. Comp Immunol Microbiol Infect Dis. 2012 Jul;35(4):309-17. doi: 10.1016/j.cimid.2012.01.012. Epub 2012 Mar 14. PubMed PMID: 22421538. (Free PMC Article available, 6 images available)
  20. Umemoto E, Otani K, Ikeno T, Verjan Garcia N, Hayasaka H, Bai Z, et al. Constitutive plasmacytoid dendritic cell migration to the splenic white pulp is cooperatively regulated by CCR7- and CXCR4-mediated signaling. J Immunol. 2012 Jul 1;189(1):191-9. doi: 10.4049/jimmunol.1200802. Epub 2012 May 25. PubMed PMID: 22634622.
  21. Le Mercier I, Poujol D, Sanlaville A, Sisirak V, Gobert M, Durand I, et al. Tumor promotion by intratumoral plasmacytoid dendritic cells is reversed by TLR7 ligand treatment. Cancer Res. 2013 Aug 1;73(15):4629-40. doi: 10.1158/0008-5472.CAN-12-3058. Epub 2013 May 30. PubMed PMID: 23722543.
  22. Hackstein H, Kranz S, Lippitsch A, Wachtendorf A, Kershaw O, Gruber AD, et al. Modulation of respiratory dendritic cells during Klebsiella pneumonia infection. Respir Res. 2013 Sep 17;14:91. doi: 10.1186/1465-9921-14-91. PubMed PMID: 24044871. (Free PMC Article available, 8 images available)
  23. Moffat JM, Segura E, Khoury G, Caminschi I, Cameron PU, Lewin SR, et al. Targeting antigen to bone marrow stromal cell-2 expressed by conventional and plasmacytoid dendritic cells elicits efficient antigen presentation. Eur J Immunol. 2013 Mar;43(3):595-605. doi: 10.1002/eji.201242799. Epub 2013 Feb 6. PubMed PMID: 23303646.
  24. Narendra SC, Chalise JP, Höök N, Magnusson M. Dendritic cells activated by double-stranded RNA induce arthritis via autocrine type I IFN signaling. J Leukoc Biol. 2014 Apr;95(4):661-6. doi: 10.1189/jlb.0613320. Epub 2013 Dec 4. PubMed PMID: 24304616.
  25. Ioannou M, Alissafi T, Boon L, Boumpas D, Verginis P. In vivo ablation of plasmacytoid dendritic cells inhibits autoimmunity through expansion of myeloid-derived suppressor cells. J Immunol. 2013 Mar 15;190(6):2631-40. doi: 10.4049/jimmunol.1201897. Epub 2013 Feb 4. PubMed PMID: 23382560. (Free PMC Article available, 8 images available)
  26. Tahiliani V, Chaudhri G, Eldi P, Karupiah G. The orchestrated functions of innate leukocytes and T cell subsets contribute to humoral immunity, virus control, and recovery from secondary poxvirus challenge. J Virol. 2013 Apr;87(7):3852-61. doi: 10.1128/JVI.03038-12. Epub 2013 Jan 23. PubMed PMID: 23345522. (Free PMC Article available, 8 images available)
  27. Sathe P, Vremec D, Wu L, Corcoran L, Shortman K. Convergent differentiation: myeloid and lymphoid pathways to murine plasmacytoid dendritic cells. Blood. 2013 Jan 3;121(1):11-9. doi: 10.1182/blood-2012-02-413336. Epub 2012 Oct 10. PubMed PMID: 23053574.
  28. Sjölin H, Robbins SH, Bessou G, Hidmark A, Tomasello E, Johansson M, et al. DAP12 signaling regulates plasmacytoid dendritic cell homeostasis and down-modulates their function during viral infection. J Immunol. 2006 Sep 1;177(5):2908-16. PubMed PMID: 16920926.
  29. Delale T, Paquin A, Asselin-Paturel C, Dalod M, Brizard G, Bates EE, et al. MyD88-dependent and -independent murine cytomegalovirus sensing for IFN-alpha release and initiation of immune responses in vivo. J Immunol. 2005 Nov 15;175(10):6723-32. PubMed PMID: 16272328.
  30. Allman D, Dalod M, Asselin-Paturel C, Delale T, Robbins SH, Trinchieri G, et al. Ikaros is required for plasmacytoid dendritic cell differentiation. Blood. 2006 Dec 15;108(13):4025-34. Epub 2006 Aug 15. PubMed PMID: 16912230. (Free PMC Article available, 7 images available)
  31. Zucchini N, Bessou G, Robbins SH, Chasson L, Raper A, Crocker PR, et al. Individual plasmacytoid dendritic cells are major contributors to the production of multiple innate cytokines in an organ-specific manner during viral infection. Int Immunol. 2008 Jan;20(1):45-56. Epub 2007 Nov 13. PubMed PMID: 18000008.
  32. Nierkens S, den Brok MH, Garcia Z, Togher S, Wagenaars J, Wassink M, et al. Immune adjuvant efficacy of CpG oligonucleotide in cancer treatment is founded specifically upon TLR9 function in plasmacytoid dendritic cells. Cancer Res. 2011 Oct 15;71(20):6428-37. doi: 10.1158/0008-5472.CAN-11-2154. Epub 2011 Jul 25. PubMed PMID: 21788345. (Free PMC Article available, 5 images available)
  33. Lahoud MH, Ahmet F, Kitsoulis S, Wan SS, Vremec D, Lee CN, et al. Targeting antigen to mouse dendritic cells via Clec9A induces potent CD4 T cell responses biased toward a follicular helper phenotype. J Immunol. 2011 Jul 15;187(2):842-50. doi: 10.4049/jimmunol.1101176. Epub 2011 Jun 15. PubMed PMID: 21677141.
  34. Cao W. Pivotal Functions of Plasmacytoid Dendritic Cells in Systemic Autoimmune Pathogenesis. J Clin Cell Immunol. 2014 Apr 22;5(2):212. PubMed PMID: 25077037. (Free PMC Article available, 2 images available)
  35. Haque A, Best SE, Montes de Oca M, James KR, Ammerdorffer A, Edwards CL, et al. Type I IFN signaling in CD8- DCs impairs Th1-dependent malaria immunity. J Clin Invest. 2014 Jun;124(6):2483-96. doi: 10.1172/JCI70698. Epub 2014 May 1. PubMed PMID: 24789914. (Free PMC Article available, 7 images available)
  36. Biessen EA, Christ A. Plasmacytoid dendritic cells in atherosclerosis: knocking at T-cell's door. Circulation. 2014 Oct 14;130(16):1340-2. doi: 10.1161/CIRCULATIONAHA.114.012641. Epub 2014 Sep 15. PubMed PMID: 25223983.
  37. Lau-Kilby AW, Kretz CC, Pechhold S, Price JD, Dorta S, Ramos H, et al. Interleukin-2 inhibits FMS-like tyrosine kinase 3 receptor ligand (flt3L)-dependent development and function of conventional and plasmacytoid dendritic cells. Proc Natl Acad Sci U S A. 2011 Feb 8;108(6):2408-13. doi: 10.1073/pnas.1009738108. Epub 2011 Jan 24. PubMed PMID: 21262836. (Free PMC Article available, 7 images available)
  38. Wykes MN, Kay JG, Manderson A, Liu XQ, Brown DL, Richard DJ, et al. Rodent blood-stage Plasmodium survive in dendritic cells that infect naive mice. Proc Natl Acad Sci U S A. 2011 Jul 5;108(27):11205-10. doi: 10.1073/pnas.1108579108. Epub 2011 Jun 20. PubMed PMID: 21690346. (Free PMC Article available, 5 images available)
  39. Lau-Kilby et al, SI, 2011
  40. Markey KA, Koyama M, Kuns RD, Lineburg KE, Wilson YA, Olver SD, et al. Immune insufficiency during GVHD is due to defective antigen presentation within dendritic cell subsets. Blood. 2012 Jun 14;119(24):5918-30. doi: 10.1182/blood-2011-12-398164. Epub 2012 Mar 13. PubMed PMID: 22415754.
  41. Alari-Pahissa E, Vega-Ramos J, Zhang JG, Castaño AR, Turley SJ, Villadangos JA, et al. Differential effect of CD69 targeting on bystander and antigen-specific T cell proliferation. J Leukoc Biol. 2012 Jul;92(1):145-58. doi: 10.1189/jlb.1011499. Epub 2012 Apr 27. PubMed PMID: 22544938.
  42. Weigert A, Weichand B, Sekar D, Sha W, Hahn C, Mora J, et al. HIF-1α is a negative regulator of plasmacytoid DC development in vitro and in vivo. Blood. 2012 Oct 11;120(15):3001-6. doi: 10.1182/blood-2012-03-417022. Epub 2012 Aug 30. PubMed PMID: 22936665.
  43. Weigert A et al, 2012, Suppl
  44. Schlitzer A, Heiseke AF, Einwächter H, Reindl W, Schiemann M, Manta CP, et al. Tissue-specific differentiation of a circulating CCR9- pDC-like common dendritic cell precursor. Blood. 2012 Jun 21;119(25):6063-71. doi: 10.1182/blood-2012-03-418400. Epub 2012 Apr 30. PubMed PMID: 22547585.
  45. Pinto A, Rega A, Crother TR, Sorrentino R. Plasmacytoid dendritic cells and their therapeutic activity in cancer. Oncoimmunology. 2012 Aug 1;1(5):726-734. PubMed PMID: 22934264. (Free PMC Article available, 2 images available)
  46. Cheong C, Choi JH. Dendritic cells and regulatory T cells in atherosclerosis. Mol Cells. 2012 Oct;34(4):341-7. doi: 10.1007/s10059-012-0128-9. Epub 2012 Jun 28. PubMed PMID: 22752759. (Free PMC Article available, 2 images available)
  47. Crother TR, Ma J, Jupelli M, Chiba N, Chen S, Slepenkin A, et al. Plasmacytoid dendritic cells play a role for effective innate immune responses during Chlamydia pneumoniae infection in mice. PLoS One. 2012;7(10):e48655. doi: 10.1371/journal.pone.0048655. Epub 2012 Oct 31. PubMed PMID: 23119083. (Free PMC Article available, 7 images available)
  48. Hackstein H, Wachtendorf A, Kranz S, Lohmeyer J, Bein G, Baal N. Heterogeneity of respiratory dendritic cell subsets and lymphocyte populations in inbred mouse strains. Respir Res. 2012 Oct 15;13:94. doi: 10.1186/1465-9921-13-94. PubMed PMID: 23066782. (Free PMC Article available, 9 images available)
  49. Willart MA, Poulliot P, Lambrecht BN, Kool M. PAMPs and DAMPs in allergy exacerbation models. Methods Mol Biol. 2013;1032:185-204. doi: 10.1007/978-1-62703-496-8_15. PubMed PMID: 23943454.
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl / 1 mg in 2ml Tris-NaCl pH8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
State:
Purified
Specificity
Specificity:
Mouse pDCs/IFN producing cells (IPC) (extracellular domain).
Species cross-reactivity: nd

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