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DDX0463P-50 SEMA6A antibody

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Quick Overview

Mouse anti Human SEMA6A 118E7

DDX0463P-50

Product Description for SEMA6A

Mouse anti Human SEMA6A 118E7.
Presentation: Purified
Product is tested for Flow Cytometry.

Properties for SEMA6A

Product Category Primary Antibodies
Quantity 50 µg
Synonyms KIAA1368, SEMA6A-1, SEMAQ, Sema VIA, Semaphorin VIA, Semaphorin-6A, Semaphorin-6A-1
Presentation Purified
Reactivity Hu
Applications F
Clonality Monoclonal
Clone 118E7
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9H2E6
Immunogen:
human recombinant SEMA-6A
GeneID:
57556
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application DDX0463P-50 / DDX0463P-100 Purified: Surface Flow Cytometry.
DDX0463A488-50 / DDX0463A488-100 Alexa-fluor®488: Surface Flow Cytometry. 
DDX0463A546-50 / DDX0463A546-100 Alexa-fluor®546: IF.
DDX0463A647-50 / DDX0463A647-100 Alexa- fluor®647: (on request) IHC, Flow cytometry. 

Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before using.
Background Semaphorins represent a large family composed of 19 secreted, GPI-linked or transmembrane members divided into 5 classes (3 to 7) and characterized by the presence of a 500 amino acid Sema domain in the Nterminus. Initially described for their role in axon guidance and apoptosis during neuronal development, some semaphorins like the class 4 regulate immune functions and are involved in organogenesis and angiogenesis. The transmembrane class 6 semaphorins include 4 members (SEMA-6A to 6D). SEMA-6A features an intracellular binding site for regulatory Evl protein. In the immune system, SEMA6A protein is restricted in vitro and in vivo to an activation status of Langerhans cells. (Gautier G et al., 2006 Am J Pathol., 168: 453-65).
Concentration 0.5 mg/ml
General Readings
  1. Gautier G, de Saint-Vis B, Sénéchal B, Pin JJ, Bates EE, Caux C, et al. The class 6 semaphorin SEMA6A is induced by interferon-gamma and defines an activation status of langerhans cells observed in pathological situations. Am J Pathol. 2006 Feb;168(2):453-65. PubMed PMID: 16436660. (Free PMC Article available, 8 images available)
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl PBS 50% glycerol.
State:
Purified
Specificity
Specificity:
Human SEMA-6A (epitope in extracellular domain).
Gene ID 57556

Accessory Products

Proteins and/or Positive Controls

Proteins for SEMA6A (3 products)

Catalog No. Species Pres. Purity   Source  

SEMA6A
SEMA6A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

SEMA6A
SEMA6A Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

SEMA6A
SEMA6A Human
  Abnova Taiwan Corp.

Positive controls for SEMA6A (3 products)

Catalog No. Species Pres. Purity   Source  

SEMA6A 293T Cell Transient Overexpression Lysate(Denatured)
SEMA6A 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

Sema6A Lysate
Western Blot: Sema6A Lysate [NBL1-15804] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for SEMA6A
  Novus Biologicals Inc.

SEMA6A overexpression lysate
SEMA6A overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

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Click here to see all secondary antibodies for SEMA6A
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