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DDX0483A546-50 CD288 / TLR8 antibody

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Rat anti Human CD288 / TLR8 112H7.15

DDX0483A546-50

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  • DDX0483A546-50

Product Description for CD288 / TLR8

Rat anti Human CD288 / TLR8 112H7.15.
Presentation: Alexa Fluor 546
Product is tested for Immunocytochemistry/Immunofluorescence.

Properties for CD288 / TLR8

Product Category Primary Antibodies
Quantity 50 µg
Synonyms Toll-like receptor 8, UNQ249/PRO286
Presentation Alexa Fluor 546
Reactivity Hu
Applications ICC/IF
Clonality Monoclonal
Clone 112H7.15
Host Rat
Isotype IgG2a
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9NR97
Immunogen:
Human recombinant TLR8.
GeneID:
51311
Application DDX0483P-50 / DDX0483P-100 Purified: ImmunoHistoChemistry frozen sections, Western Blot, FACS intracellular. 
DDX0483A488-50 / DDX0483A488-100 Alexa-fluor®488: FACS intracellular. 
DDX0483A546-50 / DDX0483A546-100 Alexa-fluor®546: ImmunoFluorescence.
DDX0483A647-50 / DDX0483A647-100 Alexa-fluor®647: FACS intracellular. 
DDX0483B-50 / DDX0483B-100 Biotin: Western Blot.

Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before using.
Background The Toll-Like Receptors (TLRs) represent a family of germline-encoded proteins, composed of C-terminal leucine-rich repeats (LRRs), and an N-terminal Toll/Interleukin-1 Receptor (TIR) domain. In humans, 10 TLRs, sharing high sequence homology, have been identified. TLRs are critical for the detection of pathogen-associated molecular patterns (PAMPs) by the innate immune system. LRRs recognize PAMPs, and signal transduction events, initiated by the TIR domain, lead to activation of transcription factors such as AP-1, IRFs and NFkB, and therefore expression of proinflammatory cytokines and costimulatory molecules. TLR7, TLR8 and TLR9 form a subgroup in the TLR family, because of a strong sequence homology and the nature of their ligands, which are nucleic acids or related molecules. TLR7 and TLR8 are triggered by GU-rich, single-stranded RNA (ssRNA) derived from viruses, or by synthetic small molecules mimicking ssRNA, such as imidazoquinolines. While TLR7 is expressed in lung, placenta and spleen, TLR8 expression is restricted to lung and peripheral blood leukocytes (PBLs). (Heil F. et al, 2003, Eur.J.Immunol, 33, 2987).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
  1. Itoh H, Tatematsu M, Watanabe A, Iwano K, Funami K, Seya T, et al. UNC93B1 physically associates with human TLR8 and regulates TLR8-mediated signaling. PLoS One. 2011;6(12):e28500. doi: 10.1371/journal.pone.0028500. Epub 2011 Dec 2. PubMed PMID: 22164301. (Free PMC Article available, 6 images available)
General Readings
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Alexa Fluor 546
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
Specificity
Specificity:
Human TLR8.
Species cross- reactivity: Negative.
Gene ID 51311

Accessory Products

Proteins and/or Positive Controls

Proteins for CD288 / TLR8 (4 products)

Catalog No. Species Pres. Purity   Source  

CD288 / TLR8
CD288 / TLR8 Human
  Abnova Taiwan Corp.

CD288 / TLR8
CD288 / TLR8 Human
  Abnova Taiwan Corp.

CD288 / TLR8
CD288 / TLR8 Human Purified
  Abnova Taiwan Corp.

CD288 / TLR8
CD288 / TLR8 Human Purified
  Abnova Taiwan Corp.

Positive controls for CD288 / TLR8 (1 products)

Catalog No. Species Pres. Purity   Source  

TLR8 overexpression lysate
TLR8 overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

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