Product Description for Carboxypeptidase M
Mouse anti Human Carboxypeptidase M M27.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.
Presentation: Purified
Product is tested for Frozen Sections, Immunoprecipitation, Flow Cytometry.
Properties for Carboxypeptidase M
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 50 µg |
| Synonyms | CPM |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | C, F, IP |
| Clonality | Monoclonal |
| Clone | M27 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Germany, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Dendritics SAS |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Application | DDX0520P-50 / DDX0520P-100 Purified: FACS surface, ImmunoHistoChemistry frozen sections, Immunoprecipitation. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using. |
| Background | Monoclonal antibody (MoAb) M27 was generated after immunization of mice with the human B-lineage acute lymphoblastic leukemia cell line Pre-ALP. Under reducing conditions, MoAb M27 precipitated a 60- kD surface-membrane molecule from Pre-ALP cells. Expression cloning of Pre-ALP cDNA showed that M27 recognizes carboxypeptidase M (CPM), a cell-surface, zinc-dependent protease known to cleave off basic C-terminal amino acids from peptide hormones. Using M27 antibody, CPM was detected only at discrete B lymphocyte developmental stages, namely on committed precursors and on germinal center cells. CPM was also expressed on mature T cells, mainly after activation. These results provide the first description of a carboxy-peptidase on lymphoid cells. In addition, CPM was found on granulocytes and monocytes but not on their progenitors. CPM was found to be expressed with CD10 and CD13 respectively on lymphoid and myeloid cells. (de Saint Vis B et al., Blood 1995,86,1098-105). |
| Concentration | 0.5 mg/ml |
| Product Citations |
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| General Readings | |
| Storage | -20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing. |
| Format |
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| Specificity |
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| Gene ID | 1368 |
Accessory Products
Proteins and/or Positive Controls
Proteins for Carboxypeptidase M (5 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP721093 |
Carboxypeptidase M (transcript variant 3) |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
10 µg /
€299.00
|
|||
| OriGene Technologies, Inc. | |||||||
| H00001368-P01-10 |
Carboxypeptidase M |
||||||
|
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001368-P01-25 |
Carboxypeptidase M |
||||||
|
|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001368-Q01-10 |
Carboxypeptidase M |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00001368-Q01-25 |
Carboxypeptidase M |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
Positive controls for Carboxypeptidase M (5 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-09438 |
Carboxypeptidase M Lysate |
||||||
![Western Blot: Carboxypeptidase M Lysate [NBL1-09438] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for CPM](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/Carboxypeptidase%20M%20Lysate-Western%20Blot-NBL1-09438-img0001.jpg) |
|||||||
| Novus Biologicals Inc. | |||||||
| H00001368-T01 |
CPM 293T Cell Transient Overexpression Lysate(Denatured) |
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|
|||||||
| Abnova Taiwan Corp. | |||||||
| LY400704 |
CPM overexpression lysate |
||||||
|
0.1 mg /
€295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
| LY405018 |
CPM overexpression lysate |
||||||
|
0.1 mg /
€295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
| LY423611 |
CPM overexpression lysate |
||||||
|
0.1 mg /
€295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for Carboxypeptidase M (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
||||||
|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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