Product Description for CD23
Mouse anti Human CD23 9P25.
Presentation: Purified
Product is tested for Functional assay, Flow Cytometry.
Presentation: Purified
Product is tested for Functional assay, Flow Cytometry.
Properties for CD23
| Product Category | Primary Antibodies |
|---|---|
| Quantity | 50 µg |
| Synonyms | CD23A, FCE2, FCER2, Fc-epsilon-RII, IGEBF, Immunoglobulin E-binding factor, Low affinity immunoglobulin epsilon Fc receptor, Lymphocyte IgE receptor |
| Presentation | Purified |
| Reactivity | Hu |
| Applications | F, FN |
| Clonality | Monoclonal |
| Clone | 9P25 |
| Host | Mouse |
| Isotype | IgG1 |
| Shipping to | Germany, USA/Canada |
| PDF datasheet | View Datasheet |
| Manufacturer | Dendritics SAS |
Datasheet Extract
| Immunogen |
|
|---|---|
| Isotype control | AM03095PU-N, SM10P (for use in human samples) |
| Application | Study of IgE binding to CD23 (Fc epsilon R2), FACS surface. Usage recommendation: *This monoclonal antibody may be used between 5-20 μg/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before using. |
| Background | CD23, also known as Fc epsilon RII, or FcεRII, is the "low affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites and is important in regulation of IgE levels. Human rIL-4 is able to induce the expression of low-affinity receptors for IgE (Fc epsilon RL/CD23) on resting B lymphocytes, as determined by the binding of either the anti Fc epsilon RL/CD23-specific mAb 25 or IgE. Stimulation of B cells with insolubilized anti-IgM antibody increases the number of cells expressing Fc epsilon RL/CD23 upon culturing with IL-4 and enhances the level of Fc epsilon RL/CD23 expression on these cells. Fc epsilon RL/CD23 induction is specific for IL-4 since IL-1 alpha, IL-2, IFN-gamma, B cell-derived B cell growth factor (BCGF), and a low-molecular-weight BCGF were ineffective. IFN-gamma strongly inhibited the induction of Fc epsilon RL/CD23 by IL-4. CD23 is a 45 kDa transmembrane glycoprotein associated with MHC Class II antigen, which belongs to the C-type lectin family. Expression of CD23 by B lymphocytes is up-regulated following mitogen or antigen activation. Soluble forms of CD23 are generated by proteolytic cleavage of the membrane molecule. (Defrance T. et al., J Exp Med, 1987,165, 1459 ; Bonnefoy J-Y., et al., 1996, Eur.Respir.J., 9, 635-665). |
| Concentration | 0.5 mg/ml |
| Product Citations |
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| Storage | -20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing. |
| Format |
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| Gene ID | 2208 |
Accessory Products
Proteins and/or Positive Controls
Proteins for CD23 (11 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| TP710039 |
CD23 (C-term DDK tag, transcript variant 1) |
||||||
|
Human |
> 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
SF9 cells |
20 µg /
€411.00
|
|||
| OriGene Technologies, Inc. | |||||||
| TP723391 |
CD23 (transcript variant 1) |
||||||
|
Human |
> 95 % Preparation: . Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
20 µg /
€230.00
|
|||
| OriGene Technologies, Inc. | |||||||
| AR10354PU-L |
CD23 |
||||||
|
Human | Purified | E. coli | ||||
| OriGene Technologies GmbH | |||||||
| AR10354PU-N |
CD23 |
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|
|
Human | Purified | E. coli | ||||
| OriGene Technologies GmbH | |||||||
| AR10354PU-S |
CD23 |
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|
|
Human | Purified | E. coli | ||||
| OriGene Technologies GmbH | |||||||
| AR31091PU-N |
CD23 |
||||||
|
|
Human | Purified | > 96 % by SDS-PAGE gel and HPLC analyses | E. coli | |||
| OriGene Technologies GmbH | |||||||
| AR31091PU-S |
CD23 |
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|
|
Human | Purified | > 96 % by SDS-PAGE gel and HPLC analyses | E. coli | |||
| OriGene Technologies GmbH | |||||||
| H00002208-G01 |
CD23 |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
| H00002208-P01-10 |
CD23 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| H00002208-P01-25 |
CD23 |
||||||
|
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. | ||||
| Abnova Taiwan Corp. | |||||||
| P6143 |
CD23 |
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|
|
Human | ||||||
| Abnova Taiwan Corp. | |||||||
Positive controls for CD23 (3 products)
| Catalog No. | Species | Pres. | Purity | Source | |||
|---|---|---|---|---|---|---|---|
| NBL1-10652 |
CD23 Lysate |
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![Western Blot: CD23 Lysate [NBL1-10652] - Western Blot experiments.
Left-Control; Right -Over-expression Lysate for FCER2](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/CD23%20Lysate-Western%20Blot-NBL1-10652-img0001.jpg) |
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| Novus Biologicals Inc. | |||||||
| H00002208-T01 |
FCER2 293T Cell Transient Overexpression Lysate(Denatured) |
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|
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| Abnova Taiwan Corp. | |||||||
| LY419592 |
FCER2 overexpression lysate |
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|
0.1 mg /
€295.00
|
||||||
| OriGene Technologies, Inc. | |||||||
Recommended Secondary Antibodies for CD23 (11 products)
| Catalog No. | Host | Clone/Iso. | Pres. | React. | Applications | ||
|---|---|---|---|---|---|---|---|
| R1253AP |
Mouse IgG (H+L chain) antibody |
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|
|
Rabbit | AP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253B |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Biotin | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253F |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | FITC | E, ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1253HRP |
Mouse IgG (H+L chain) antibody |
||||||
|
Rabbit | HRP | C, E, WB | ||||
| OriGene Technologies GmbH | |||||||
| R1253TR |
Mouse IgG (H+L chain) antibody |
||||||
|
|
Rabbit | Texas Red | ICC/IF | ||||
| OriGene Technologies GmbH | |||||||
| R1403R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Rabbit | PE | F | ||||
| OriGene Technologies GmbH | |||||||
| R1405R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Goat | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1455R |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody |
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|
|
Donkey | PE | |||||
| OriGene Technologies GmbH | |||||||
| R1457C2 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy2 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C3 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy3 | |||||
| OriGene Technologies GmbH | |||||||
| R1457C5 |
Mouse IgG (H+L chain), adsorbed antibody |
||||||
|
|
Goat | Cy5 | |||||
| OriGene Technologies GmbH | |||||||
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