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DDX0700A488-50 CD127 / IL7R antibody

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Mouse anti Canine, Human CD127 / IL7R R34-34

DDX0700A488-50

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  • DDX0700A488-50
  • DDX0700A488-50

Product Description for CD127 / IL7R

Mouse anti Canine, Human CD127 / IL7R R34-34.
Presentation: Alexa Fluor 488
Product is tested for Flow Cytometry.

Properties for CD127 / IL7R

Product Category Primary Antibodies
Quantity 50 µg
Synonyms IL-7R-alpha, IL7 receptor alpha, Interleukin-7 receptor subunit alpha
Presentation Alexa Fluor 488
Reactivity Can, Hu
Applications F
Clonality Monoclonal
Clone R34-34
Host Mouse
Isotype IgG1
Shipping to Germany, USA/Canada
PDF datasheet View Datasheet
Manufacturer Dendritics SAS

Datasheet Extract

Immunogen
Swiss Prot Num:
P16871
Immunogen:
Human leukemic pre-B cells.
GeneID:
3575
Application DDX0700P-50 / DDX0700P-100 Purified: FACS surface, Neutralization (IL7- binding inhibition), Immunoprecipitation.
DDX0700A488-50 / DDX0700A488-100 Alexa-fluor®488: Surface Flow cytometry.
DDX0700A546-50 / DDX0700A546-100 Alexa-fluor®546: IF.
DDX0700A647-50 / DDX0700A647-100 Alexa-fluor®647: Surface Flow cytometry.

Usage recommendation:
*This monoclonal antibody may be used between 5-20 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before using.
Background The IL7-R consists of 2 chains, IL-7Rα known as CD127 and common cytokine receptor γ chain known as CD132. A 75 to 80kDa human IL-7 receptor has been cloned that belongs to hematopoietic cytokinereceptor super family. R34-34, raised against human leukemic pre-B cells, recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7Rα expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+ memory T cells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve and memory T cells. (Pandrau-Garcia D et al, 1994, Blood, 83, 3613-9 ; Mazzucchelli R et al, Nat. Review Immunol., 2007,7, 144-54).
Concentration 0.5 mg/ml
Product Citations
Originator or purchased from resellers:
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  4. Scupoli MT, Perbellini O, Krampera M, Vinante F, Cioffi F, Pizzolo G. Interleukin 7 requirement for survival of T-cell acute lymphoblastic leukemia and human thymocytes on bone marrow stroma. Haematologica. 2007 Feb;92(2):264-6. PubMed PMID: 17296584.
  5. Gopala Krishna. Yakala. Characterization of differentially spliced interleukin-7 and interleukin-7 receptor isoforms: paradigms for alternative splicing in the immune system. Yakala GK et al, 2007
  6. Hartigan-O'Connor DJ, Poon C, Sinclair E, McCune JM. Human CD4+ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells. J Immunol Methods. 2007 Jan 30;319(1-2):41-52. Epub 2006 Nov 3. PubMed PMID: 17173927.
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  17. Sauce D, Larsen M, Abbott RJ, Hislop AD, Leese AM, Khan N, et al. Upregulation of interleukin 7 receptor alpha and programmed death 1 marks an epitope-specific CD8+ T-cell response that disappears following primary Epstein-Barr virus infection. J Virol. 2009 Sep;83(18):9068-78. doi: 10.1128/JVI.00141-09. Epub 2009 Jul 15. PubMed PMID: 19605492. (Free PMC Article available, 6 images available)
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  20. Presicce P, Moreno-Fernandez ME, Lages CS, Orsborn KI, Chougnet CA. Association of two clones allows for optimal detection of human FOXP3. Cytometry A. 2010 Jun;77(6):571-9. doi: 10.1002/cyto.a.20875. PubMed PMID: 20162533. (Free PMC Article available, 5 images available)
  21. Crawley AM, Faucher S, Angel JB. Soluble IL-7R alpha (sCD127) inhibits IL-7 activity and is increased in HIV infection. J Immunol. 2010 May 1;184(9):4679-87. doi: 10.4049/jimmunol.0903758. Epub 2010 Mar 19. PubMed PMID: 20304824.
  22. van Roon JA, Hartgring SA, van der Wurff-Jacobs KM, Bijlsma JW, Lafeber FP. Numbers of CD25+Foxp3+ T cells that lack the IL-7 receptor are increased intra-articularly and have impaired suppressive function in RA patients. Rheumatology (Oxford). 2010 Nov;49(11):2084-9. doi: 10.1093/rheumatology/keq237. Epub 2010 Aug 6. PubMed PMID: 20693259.
  23. Engram JC, Cervasi B, Borghans JA, Klatt NR, Gordon SN, Chahroudi A, et al. Lineage-specific T-cell reconstitution following in vivo CD4+ and CD8+ lymphocyte depletion in nonhuman primates. Blood. 2010 Aug 5;116(5):748-58. doi: 10.1182/blood-2010-01-263814. Epub 2010 May 18. PubMed PMID: 20484087. (Free PMC Article available, 7 images available)
  24. Valencia J, Hernández-López C, Martínez VG, Hidalgo L, Zapata AG, Vicente A, et al. Transient beta-catenin stabilization modifies lineage output from human thymic CD34+CD1a- progenitors. J Leukoc Biol. 2010 Mar;87(3):405-14. doi: 10.1189/jlb.0509344. Epub 2009 Dec 1. PubMed PMID: 19952356.
  25. Zonios DI, Falloon J, Bennett JE, Shaw PA, Chaitt D, Baseler MW, et al. Idiopathic CD4+ lymphocytopenia: natural history and prognostic factors. Blood. 2008 Jul 15;112(2):287-94. doi: 10.1182/blood-2007-12-127878. Epub 2008 May 2. PubMed PMID: 18456875. (Free PMC Article available, 4 images available)
  26. Pankewycz O, Leca N, Kohli R, Wallace PK, Said M, Feng L, et al. Low-dose rabbit antithymocyte globulin induction therapy results in prolonged selective lymphocyte depletion irrespective of maintenance immunosuppression. Transplant Proc. 2011 Mar;43(2):462-5. doi: 10.1016/j.transproceed.2011.01.034. PubMed PMID: 21440734.
  27. Mjösberg JM, Trifari S, Crellin NK, Peters CP, van Drunen CM, Piet B, et al. Human IL-25- and IL-33-responsive type 2 innate lymphoid cells are defined by expression of CRTH2 and CD161. Nat Immunol. 2011 Sep 11;12(11):1055-62. doi: 10.1038/ni.2104. PubMed PMID: 21909091.
  28. Petrovas C, Yamamoto T, Gerner MY, Boswell KL, Wloka K, Smith EC, et al. CD4 T follicular helper cell dynamics during SIV infection. J Clin Invest. 2012 Sep;122(9):3281-94. doi: 10.1172/JCI63039. Epub 2012 Aug 27. PubMed PMID: 22922258. (Free PMC Article available, 7 images available)
  29. Noakes PS, Vlachava M, Kremmyda LS, Diaper ND, Miles EA, Erlewyn-Lajeunesse M, et al. Increased intake of oily fish in pregnancy: effects on neonatal immune responses and on clinical outcomes in infants at 6 mo. Am J Clin Nutr. 2012 Feb;95(2):395-404. doi: 10.3945/ajcn.111.022954. Epub 2012 Jan 4. PubMed PMID: 22218160.
  30. Luckashenak N, Wähe A, Breit K, Brakebusch C, Brocker T. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol. 2013 Jan 1;190(1):27-35. doi: 10.4049/jimmunol.1201082. Epub 2012 Dec 3. PubMed PMID: 23209325.
  31. Magalhaes I et al, 2012
  32. Puronen CE, Thompson WL, Imamichi H, Beq S, Hodge JN, Rehm C, et al. Decreased interleukin 7 responsiveness of T lymphocytes in patients with idiopathic CD4 lymphopenia. J Infect Dis. 2012 May 1;205(9):1382-90. doi: 10.1093/infdis/jis219. Epub 2012 Mar 26. PubMed PMID: 22454463. (Free PMC Article available, 5 images available)
  33. Yatim A, Benne C, Sobhian B, Laurent-Chabalier S, Deas O, Judde JG, et al. NOTCH1 nuclear interactome reveals key regulators of its transcriptional activity and oncogenic function. Mol Cell. 2012 Nov 9;48(3):445-58. doi: 10.1016/j.molcel.2012.08.022. Epub 2012 Sep 27. PubMed PMID: 23022380. (Free PMC Article available, 7 images available)
  34. Yatim A et al, SI, 2012
  35. Young CD et al, 2012
  36. Ponchel F, Goëb V, Parmar R, El-Sherbiny Y, Boissinot M, El Jawhari J, et al. An immunological biomarker to predict MTX response in early RA. Ann Rheum Dis. 2014 Nov;73(11):2047-53. doi: 10.1136/annrheumdis-2013-203566. Epub 2013 Aug 29. PubMed PMID: 23989988.
  37. Compeer EB, Flinsenberg TW, Boon L, Hoekstra ME, Boes M. Tubulation of endosomal structures in human dendritic cells by Toll-like receptor ligation and lymphocyte contact accompanies antigen cross-presentation. J Biol Chem. 2014 Jan 3;289(1):520-8. doi: 10.1074/jbc.M113.511147. Epub 2013 Nov 14. PubMed PMID: 24235148. (Free PMC Article available)
  38. Oliveira L et al, 2013
  39. Ghazawi FM, Faller EM, Sugden SM, Kakal JA, MacPherson PA. IL-7 downregulates IL-7Rα expression in human CD8 T cells by two independent mechanisms. Immunol Cell Biol. 2013 Feb;91(2):149-58. doi: 10.1038/icb.2012.69. Epub 2012 Dec 4. PubMed PMID: 23207282.
  40. Yu X, Pappu R, Ramirez-Carrozzi V, Ota N, Caplazi P, Zhang J, et al. TNF superfamily member TL1A elicits type 2 innate lymphoid cells at mucosal barriers. Mucosal Immunol. 2014 May;7(3):730-40. doi: 10.1038/mi.2013.92. Epub 2013 Nov 13. PubMed PMID: 24220298. (Free PMC Article available, 6 images available)
  41. Bernink JH, Peters CP, Munneke M, te Velde AA, Meijer SL, Weijer K, et al. Human type 1 innate lymphoid cells accumulate in inflamed mucosal tissues. Nat Immunol. 2013 Mar;14(3):221-9. doi: 10.1038/ni.2534. Epub 2013 Jan 20. PubMed PMID: 23334791.
  42. Forsberg A et al, 2014
  43. Faller EM, McVey MJ, MacPherson PA. IL-7 receptor recovery on CD8 T-cells isolated from HIV+ patients is inhibited by the HIV Tat protein. PLoS One. 2014 Jul 17;9(7):e102677. doi: 10.1371/journal.pone.0102677. eCollection 2014. PubMed PMID: 25033393. (Free PMC Article available, 3 images available)
  44. Larbi A, Mitjavila-Garcia MT, Flamant S, Valogne Y, Clay D, Usunier B, et al. Generation of multipotent early lymphoid progenitors from human embryonic stem cells. Stem Cells Dev. 2014 Dec 15;23(24):2983-95. doi: 10.1089/scd.2014.0171. PubMed PMID: 24955741.
  45. Munneke JM, Björklund AT, Mjösberg JM, Garming-Legert K, Bernink JH, Blom B, et al. Activated innate lymphoid cells are associated with a reduced susceptibility to graft-versus-host disease. Blood. 2014 Jul 31;124(5):812-21. doi: 10.1182/blood-2013-11-536888. Epub 2014 May 22. PubMed PMID: 24855210.
  46. Sereti I, Estes JD, Thompson WL, Morcock DR, Fischl MA, Croughs T, et al. Decreases in colonic and systemic inflammation in chronic HIV infection after IL-7 administration. PLoS Pathog. 2014 Jan 30;10(1):e1003890. doi: 10.1371/journal.ppat.1003890. eCollection 2014 Jan. PubMed PMID: 24497828. (Free PMC Article available, 6 images available)
  47. Péguillet I, Milder M, Louis D, Vincent-Salomon A, Dorval T, Piperno-Neumann S, et al. High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer. Cancer Res. 2014 Apr 15;74(8):2204-16. doi: 10.1158/0008-5472.CAN-13-2269. Epub 2014 Feb 17. PubMed PMID: 24535711.
General Readings
Storage -20°C. KEEP CONTENTS STERILE: no preservative.
Purified antibodies: avoid repeated freeze/thaw cycles.
Coupled antibodies: glycerol protects from freezing.
Format
Label:
Alexa Fluor 488
Purification:
QMA Hyper D ion exchange chromatography
Buffer System:
Purified: 100 μg in 200μl / 50 μg in 100 μl Tris-NaCl pH 8.
Coupled: 100 μg in 200μl / 50 μg in 100 μl Tris PBS 50% glycerol.
Specificity
Specificity:
Human IL7-Rα.
Gene ID 3575

Accessory Products

Proteins and/or Positive Controls

Proteins for CD127 / IL7R (6 products)

Catalog No. Species Pres. Purity   Source  

CD127 / IL7R
CD127 / IL7R Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

CD127 / IL7R
CD127 / IL7R Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
10 µg / €299.00
  OriGene Technologies, Inc.

CD127 / IL7R
CD127 / IL7R Human
  Abnova Taiwan Corp.

CD127 / IL7R
CD127 / IL7R Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD127 / IL7R
CD127 / IL7R Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

CD127 / IL7R
Western Blot: IL7 Receptor alpha Protein [NBP1-46087] - Lane 1 – MW markers; Lane 2 – IL-7 R alpha-Fc Chimera; Lane 3 – IL-7 R alpha-Fc Chimera treated with PNGase F to remove potential N-linked glycans. 10 µg protein loaded per lane; Deep Purple™ stained. Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. Bands in lane 3 at 35 kDa is PNGase F protein. Human Purified
  Novus Biologicals Inc.

Positive controls for CD127 / IL7R (3 products)

Catalog No. Species Pres. Purity   Source  

IL7R 293T Cell Transient Overexpression Lysate(Denatured)
IL7R 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

IL7 Receptor alpha Lysate
Western Blot: IL7 Receptor alpha Lysate [NBL1-11966] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for IL7R.
  Novus Biologicals Inc.

IL7R overexpression lysate
IL7R overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

Recommended Secondary Antibodies for CD127 / IL7R (11 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
    OriGene Technologies GmbH
Click here to see all secondary antibodies for CD127 / IL7R
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