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AM26074PU-N HIV-1 Nef / HXB2 (31-35) antibody

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0.1 mg / €420.00
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Quick Overview

Mouse anti Human HIV-1 3F2 (01-001)

AM26074PU-N

Product Description for HIV-1

Mouse anti Human HIV-1 3F2 (01-001).
Properties: Nef / HXB2 (31-35)
Presentation: Aff - Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for HIV-1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms HIV-I, HIV1, Human immunodeficiency virus type 1
Presentation Aff - Purified
Reactivity Hu
Applications C, E, IP, WB
Clonality Monoclonal
Clone 3F2 (01-001)
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Recombinant Nef protein of HIV-1 HXB2 (purified from E. coli)
Property 31-35, Nef / HXB2
Application Reacts in ELISA, Western blot, Immunoprecipitation and Immunohistochemistry on Frozen Sections with HIV-1 Nef of BRU, IIIB, LAV, HAN strains.
Titer using rNef protein with subtype C a.a. consensus:
ELISA: 1/5000.
Western blot: 1/1000.
Background HIV is a highly variable virus which mutates very readily. This means there are many different strains of HIV, even within the body of a single infected person. The strains of HIV1 can be classified into three groups: the "major" group M, the "outlier" group O and the "new" group N. These three groups may represent three separate introductions of simian immunodeficiency virus into humans. Group O appears to be restricted to West-Central Africa and group N, discovered in 1998 in Cameroon, is extremely rare. More than 90% of HIV1 infections belong to HIV1 group M.
Concentration 1.0 mg/ml
General Readings
  1. Ovod V, Lagerstedt A, Ranki A, Gombert FO, Spohn R, Tähtinen M, et al. Immunological variation and immunohistochemical localization of HIV-1 Nef demonstrated with monoclonal antibodies. AIDS. 1992 Jan;6(1):25-34. PubMed PMID: 1371924.
  2. Saito Y, Sharer LR, Epstein LG, Michaels J, Mintz M, Louder M, et al. Overexpression of nef as a marker for restricted HIV-1 infection of astrocytes in postmortem pediatric central nervous tissues. Neurology. 1994 Mar;44(3 Pt 1):474-81. PubMed PMID: 8145918.
  3. Ranki A, Nyberg M, Ovod V, Haltia M, Elovaara I, Raininko R, et al. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS. 1995 Sep;9(9):1001-8. PubMed PMID: 8527071.
  4. Collings A, Pitkänen J, Strengell M, Tähtinen M, Lagerstedt A, Hakkarainen K, et al. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine. 1999 Oct 14;18(5-6):460-7. PubMed PMID: 10519935.
  5. Kjerrström A, Hinkula J, Engström G, Ovod V, Krohn K, Benthin R, et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology. 2001 May 25;284(1):46-61. PubMed PMID: 11352667.
  6. Blazevic V, Männik A, Malm M, Sikut R, Valtavaara M, Toots U, et al. Induction of human immunodeficiency virus type-1-specific immunity with a novel gene transport unit (GTU)-MultiHIV DNA vaccine. AIDS Res Hum Retroviruses. 2006 Jul;22(7):667-77. PubMed PMID: 16831091.
Storage Upon receipt, store (in aliquots) at -20 to -70 °C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography on Protein G
Buffer System:
PBS, pH 7.4
Preservatives:
0.09% Sodium Azide
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
This antibody detects HIV-1 Nef regulatory protein, mapped to amino acids 31-35 (-GAASR-, HIV-1 HXB2).
Reacts with HIV-1 subtype C Nef of BRU, IIIB, LAV, HAN strains.

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