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AM26082PU-N HIV-1 Tat (2-9) antibody

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0.1 mg / €420.00
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Quick Overview

Mouse anti Human HIV-1 1 (02-001)

AM26082PU-N

Product Description for HIV-1

Mouse anti Human HIV-1 1 (02-001).
Properties: Tat (2-9)
Presentation: Aff - Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunoprecipitation, Western blot / Immunoblot.

Properties for HIV-1

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms HIV-I, HIV1, Human immunodeficiency virus type 1
Presentation Aff - Purified
Reactivity Hu
Applications C, E, IP, WB
Clonality Monoclonal
Clone 1 (02-001)
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Property 2-9, Tat
Application Reacts in ELISA, Western blot, Immunoprecipitation, and Immunohistochemistry on frozen sections with HIV-1 Tat of HXB2, IIIB, HAN strains. Titer using rTat protein HXB2:
ELISA: 1:5000.
Western blot: 1:1000.
Background HIV is a highly variable virus which mutates very readily. This means there are many different strains of HIV, even within the body of a single infected person. The strains of HIV1 can be classified into three groups: the "major" group M, the "outlier" group O and the "new" group N. These three groups may represent three separate introductions of simian immunodeficiency virus into humans. Group O appears to be restricted to West-Central Africa and group N, discovered in 1998 in Cameroon, is extremely rare. More than 90% of HIV1 infections belong to HIV1 group M.
Concentration 1.0 mg/ml
General Readings
  1. Ranki A, Lagerstedt A, Ovod V, Aavik E, Krohn KJ. Expression kinetics and subcellular localization of HIV-1 regulatory proteins Nef, Tat and Rev in acutely and chronically infected lymphoid cell lines. Arch Virol. 1994;139(3-4):365-78. PubMed PMID: 7832642.
  2. Ranki A, Nyberg M, Ovod V, Haltia M, Elovaara I, Raininko R, et al. Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS. 1995 Sep;9(9):1001-8. PubMed PMID: 8527071.
  3. Tähtinen M, Ranki A, Valle SL, Ovod V, Krohn K. B-cell epitopes in HIV-1 Tat and Rev proteins colocalize with T-cell epitopes and with functional domains. Biomed Pharmacother. 1997;51(10):480-7. PubMed PMID: 9863510.
  4. Henderson WW, Ruhl R, Lewis P, Bentley M, Nelson JA, Moses AV. Human immunodeficiency virus (HIV) type 1 Vpu induces the expression of CD40 in endothelial cells and regulates HIV-induced adhesion of B-lymphoma cells. J Virol. 2004 May;78(9):4408-20. PubMed PMID: 15078922. (Free PMC Article available, 5 images available)
  5. Kjerrström A, Hinkula J, Engström G, Ovod V, Krohn K, Benthin R, et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology. 2001 May 25;284(1):46-61. PubMed PMID: 11352667.
Storage Upon receipt, store (in aliquots) at -20 to -70 °C. Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity chromatography
Buffer System:
PBS pH 7.4, with 0.09% sodium azide
State:
Liquid Ig fraction
Aff - Purified
Specificity
Specificity:
This antibody detects HIV-1 Tat (aa 2-9).
It recognizes the rTat consensus sequences of HIV-1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE).

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