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AM26100PU-N BPV-1 E2 tag (GVSSTSSDFRDR) antibody

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0.1 mg / €420.00
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Quick Overview

Mouse anti Bovine BPV-1 E2 tag 3F12

AM26100PU-N

Product Description for BPV-1 E2 tag

Mouse anti Bovine BPV-1 E2 tag 3F12.
Properties: (GVSSTSSDFRDR)
Presentation: Aff - Purified
Product is tested for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for BPV-1 E2 tag

Product Category Primary Antibodies
Quantity 0.1 mg
Synonyms BPV1, Bovine Papilloma Virus Protein E2
Presentation Aff - Purified
Reactivity Bov
Applications ICC/IF, IP, WB
Clonality Monoclonal
Clone 3F12
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P03122
Immunogen:
Recombinant full length BPV type 1 E2 protein purified from E. coli
Property GVSSTSSDFRDR
Application Western blot (1/1000–1/5000): Dilute to a concentration of 1.0-0.2 μg/ml in a diluent containing 2% non-fat dry milk in TBST (Tris-Buffered Saline, Tween-20). (Background can be reduced by adding NaCl up to a concentration of 1.5 M.) Incubate the membrane with diluted anti-E2Tag antibody for 1 hour at room temperature with gentle orbital shaking.
Immunofluorescence (1/1000): Dilute anti-E2Tag antibody to a
concentration of 1-2 μg/ml in a diluent containing 1% BSA in PBS. Incubate with diluted anti-E2Tag antibody for 1 hour at room temperature.
Immunoprecipitation.
This antibody belongs to the IgG1 subclass, thus, we recommend using protein G-agarose for Binding experiments.
Background The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. Binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. Can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
Concentration 1.0 mg/ml
General Readings
  1. Kurg R, Parik J, Juronen E, Sedman T, Abroi A, Liiv I, et al. Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. J Virol. 1999 Jun;73(6):4670-7. PubMed PMID: 10233926. (Free PMC Article available, 5 images available)
  2. Kaldalu N, Lepik D, Kristjuhan A, Ustav M. Monitoring and purification of proteins using bovine papillomavirus E2 epitope tags. Biotechniques. 2000 Mar;28(3):456-60, 462. PubMed PMID: 10723557.
  3. Wu K, Liu M, Li A, Donninger H, Rao M, Jiao X, et al. Cell fate determination factor DACH1 inhibits c-Jun-induced contact-independent growth. Mol Biol Cell. 2007 Mar;18(3):755-67. Epub 2006 Dec 20. PubMed PMID: 17182846. (Free PMC Article available, 10 images available)
Storage Upon receipt, store (in aliquots) at -20°C to -70°C.
Avoid repeated freezing and thawing.
Shelf life: One year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS with 50% Glycerol
State:
Liquid purified Ig fraction
Aff - Purified
Specificity
Specificity:
This antibody detects Bovine Papillomavirus E2-protein mapped to E2Tag (aa 197-208, GVSSTSSDFRDR).
Isolates E2Tag-tagged proteins and associated proteins/protein binding partners by Immunoprecipitation.

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