AR09956PU-N ALKBH2 / ABH2 (1-261, His-tag)

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0.1 mg / €320.00
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ALKBH2 / ABH2

AR09956PU-N

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  • AR09956PU-N

Product Description for ALKBH2 / ABH2

ALKBH2 / ABH2.
Presentation: Purified

Properties for ALKBH2 / ABH2

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms Alkylated DNA repair protein alkB homolog 2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, Oxy DC1
Presentation Purified
Molecular weight 31.4 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q6NS38
Purity > 90 %
Background Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and α-ketoglutarate for enzymatic activity.
Concentration 0.5 mg/ml (determined by Bradford assay)
General Readings Rinqvoll J., et al. (2008) Cancer Res. 68:4142-4149.
Lee D H., et al. (2005) J Biol Chem. 280:39448-39459.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human ALKBH2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K
Molecular weight:
(281aa), confirmed by MALDI-TOF
Format
Buffer System:
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol
Purity:
>90%
State:
Liquid purified protein
Purified
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