EEF1E1 (1-174, His-tag) - AR09962PU-L

Product Description for EEF1E1

EEF1E1.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.5 mg
Synonyms	AIMP3, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Elongation factor p18, Eukaryotic translation elongation factor 1 epsilon-1, Multisynthetase complex auxiliary component p18, P18
Presentation	Purified
Molecular weight	21.9 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: O43324
Purity	> 95 %
Background	EEF1E1, also known as eukaryotic translation elongation factor 1 epsilon-1, shares sequence similarity with the amino-terminal ends of the Beta and Gamma subunits of EF-1. By specifically interacting with MetRS, this protein binds to a macromolecular tRNA synthtase complex that catalyzes the ligation of specific amino acids to their cognate tRNAs. Upon DNA damage, it translocates to the nucleus where it interacts with ATM and ATR, resulting in p53 activation.
Concentration	1.0 mg/ml (determined by Bradford assay)
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human EEIF1E1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography AA Sequence: `MGSSHHHHHH SSGLVPRGSH MAAAAELSLL EKSLGLSKGN KYSAQGERQI PVLQTNNGPS LTGLTTIAAH LVKQANKEYL LGSTAEEKAI VQQWLEYRVT QVDGHSSKND IHTLLKDLNS YLEDKVYLTG YNFTLADILL YYGLHRFIVD LTVQEKEKYL NVSRWFCHIQ HYPGIRQHLS SVVFIKNRLY TNSH` Molecular weight: (194aa) confirmed by MALDI-TOF
Format	Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT Purity: >95% State: Liquid purified protein Purified