hchA (1-283, His-tag) - AR09963PU-L

Product Description for hchA

hchA.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.5 mg
Synonyms	Chaperone protein hchA, EcHsp31, Hsp31, yedU, yzzC
Presentation	Purified
Molecular weight	33.3 kDa
Source	E. coli
Species (Protein)	E. coli
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P31658
Purity	> 95 %
Background	HchA, also known as, Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress.
Concentration	1.0 mg/ml (determined by Bradford assay)
General Readings	Malki A. et al. (2003) Biochem. Biophys. Res. Commun. 301:430-436.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant E. coli hchA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MTVQTSKNPQ VDIAEDNAFF PSEYSLSQYT SPVSDLDGVD YPKPYRGKHK ILVIAADERY LPTDNGKLFS TGNHPIETLL PLYHLHAAGF EFEVATISGL MTKFEYWAMP HKDEKVMPFF EQHKSLFRNP KKLADVVASL NADSEYAAIF VPGGHGALIG LPESQDVAAA LQWAIKNDRF VISLCHGPAA FLALRHGDNP LNGYSICAFP DAADKQTPEI GYMPGHLTWY FGEELKKMGM NIINDDITGR VHKDRKLLTG DSPFAANALG KLAAQEMLAA YAG` Molecular weight: (303aa) confirmed by MALDI-TOF
Format	Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT Purity: >95% State: Liquid purified protein Purified