Endonuclease III / nth (1-211, His-tag) - AR09992PU-N

Product Description for Endonuclease III / nth

Endonuclease III / nth.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	50 µg
Synonyms	DNA-(apurinic or apyrimidinic site) lyase, JW1625, b1633
Presentation	Purified
Molecular weight	25.7 kDa
Source	E. coli
Species (Protein)	E. coli
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: P0AB83
Purity	> 85 %
Background	Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
Concentration	0.5 mg/ml (determined by Bradford assay)
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant E.coli nth protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I` Molecular weight: (231aa) confirmed by MALDI-TOF
Format	Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF Purity: >85% State: Liquid purified protein Purified