AR09995PU-L YARS (1-528, His-tag)

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0.25 mg / €1,070.00
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YARS

AR09995PU-L

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  • AR09995PU-L

Product Description for YARS

YARS.
Presentation: Purified

Properties for YARS

Product Category Proteins & Growth Factors
Quantity 0.25 mg
Synonyms TyrRS, Tyrosyl-tRNA ligase, Tyrosyl-tRNA synthetase cytoplasmic
Presentation Purified
Molecular weight 61.3 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P54577
Purity > 90 %
Background Tyrosyl-tRNA synthetase, also known as YARS, belongs to the class I tRNA synthetase family. YARS is essential enzyme that catalyze the aminoacylation of tRNATrp with tryptophan, an essential function of the cell’s protein synthesis machinery. Expression of YARS is highly stimulated in human cells by the addition of IFN-gamma.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Kise Y., et al. (2004) Nat Struct Mol Biol. 11:149-156.
Wakasugi K., et al. (2002) Proc Natl Acad Sci . 99:173-177.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant human YARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKIYW GTATTGKPHV AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVSYYENV IKAMLESIGV PLEKLKFIKG TDYQLSKEYT LDVYRLSSVV TQHDSKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC EPGNVENNGV LSFIKHVLFP LKSEFVILRD EKWGGNKTYT AYVDLEKDFA AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPM AKGPAKNSEP EEVIPSRLDI RVGKIITVEK HPDADSLYVE KIDVGEAEPR TVVSGLVQFV PKEELQDRLV VVLCNLKPQK MRGVESQGML LCASIEGINR QVEPLDPPAG SAPGEHVFVK GYEKGQPDEE LKPKKKVFEK LQADFKISEE CIAQWKQTNF MTKLGSISCK SLKGGNIS
Molecular weight:
(548aa)
Format
Buffer System:
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Purity:
>90%
State:
Liquid purified protein
Purified
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