AP31521SU-N Complement C3c antibody

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1 ml / €170.00
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Quick Overview

Rabbit anti Human Complement C3c

AP31521SU-N

Product Description for Complement C3c

Rabbit anti Human Complement C3c.
Presentation: Serum
Product is tested for Paraffin Sections, Immunodiffusion, Immunoprecipitation.

Properties for Complement C3c

Product Category Primary Antibodies
Quantity 1 ml
Synonyms CPAMD1, Complement component 3
Presentation Serum
Reactivity Hu
Applications ID, IP, P
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P01026
Immunogen:
C3c is the major fragment resulting from the C3 cleavage by C3 convertase and factor i. It is composed of an intact beta chain bound to two fragments of the alpha chain. The protein is isolated and purified from pooled normal human serum by precipitation techniques, followed by chromatographical methods.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
24232
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Can be used in precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of complement C3c or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibody-binding techniques if proper controls are included. Determinations of individual complement components can be very useful in defining the exact location of a defect.
Also suitable for Immunohistochemistry on Paraffin Sections.
Directions for use:
In immunoelectrophoresis use 2 μl human plasma or equivalent against 120 μl antiserum.
In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
In single radial immunodiffusion use 1 percent antiserum in the gel. 
Immunohistochemistry on Paraffin Sections: 1/200.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
No preservative added. - No foreign proteins added.
State:
Delipidated, heat inactivated, lyophilized, stable whole serum.
Serum
Reconstitution:
Restore with 1 ml sterile distilled water.
Specificity
Specificity:
In immunoelectrophoresis against fresh human serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c can also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of human serum or plasma.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.
Species:
Human.
Gene ID 717

Accessory Products

Proteins and/or Positive Controls

Proteins for Complement C3c (3 products)

Catalog No. Species Pres. Purity   Source  

Complement C3c

Complement C3c Human Purified > 96.0%
  OriGene Technologies GmbH

Complement C3c

Complement C3c Human Purified > 96.0%
  OriGene Technologies GmbH

Complement C3c

Complement C3c Human Purified > 96.0%
  OriGene Technologies GmbH
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