AP31522SU-N Complement C4A/C4B antibody

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1 ml / €230.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Rabbit anti Human Complement C4A/C4B

AP31522SU-N

Product Description for Complement C4A/C4B

Rabbit anti Human Complement C4A/C4B.
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Complement C4A/C4B

Product Category Primary Antibodies
Quantity 1 ml
Synonyms Acidic complement C4, Basic complement C4, CPAMD2, CPAMD3, Complement component 4
Presentation Serum
Reactivity Hu
Applications ID, IP
Clonality Polyclonal
Host Rabbit
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0C0L4
Immunogen:
C4 and C2 represent substrates for activated C1. Both components are present in serum in an inert form. C4 is composed of 3 polypeptide chains of three different types, , , . One of the chains is cleaved by the action of activated C1, resulting in activation of C4 exposing an binding site for the next component in the sequence, C2. C4 has a molecular weight of 205.000 and is present in plasma in an average concentration of 600 μg/ml. The activation of C4 yields two peptide fragments: C4a (MW 8,000) and C4b (MW 198,000). This process shows a considerable degree of similarity to the activation of the components C3 and C5. The C4 is isolated as a homogenous protein for use in antiserum production. Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
720
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Can be used in precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of C4 containing complexes in human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive nonprecipitating antibody-binding techniques if proper controls are included.
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Buffer System:
No preservative added. - No foreign proteins added.
State:
Delipidated, heat inactivated, lyophilized, stable whole antiserum
Serum
Reconstitution:
Restore with 1 ml sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is restricted to C4. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C4 protein. Activated C4b may also react with the antiserum. No reaction is obtained with any other plasma protein component or serum.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum has been tested for cross-reactivity in double radial immunodiffusion with bovine serum and found negative.
Species:
Human.
Gene ID 717

Accessory Products

Proteins and/or Positive Controls

Proteins for Complement C4A/C4B (9 products)

Catalog No. Species Pres. Purity   Source  

Complement C4A/C4B

Complement C4A/C4B Human
  Abnova Taiwan Corp.

Complement C4A/C4B

Complement C4A/C4B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Complement C4A/C4B

Complement C4A/C4B Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Complement C4A/C4B

Complement C4A/C4B Human > 99 % Serum
  BBI Solutions

Complement C4A/C4B

Complement C4A/C4B Human > 96 % Serum
  BBI Solutions

Complement C4c

Complement C4c Human Purified > 95 % Affinity chromatography - SDS PAGE: >95%
1 mg / €620.00
  OriGene Technologies GmbH

Complement C4c

Complement C4c Human Purified > 99.0%
  OriGene Technologies GmbH

Complement C4c

Complement C4c Human Purified > 99.0%
  OriGene Technologies GmbH

Complement C4c

Complement C4c Human Purified > 99.0%
  OriGene Technologies GmbH
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