Human Lambda Light Chain (free) - AP31533SU-N

Product Description for Human Lambda Light Chain

Goat anti Human Human Lambda Light Chain.
Properties: (free)
Presentation: Serum
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Human Lambda Light Chain

Product Category	Primary Antibodies
Quantity	1 ml
Presentation	Serum
Reactivity	Hu
Applications	ID, IP
Clonality	Polyclonal
Host	Goat
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Immunogen: A pool of purified Bence Jones lambda proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Property	free
Application	To detect and identify free light chain type and Bence Jones protein in serum or urine by immunoelectrophoresis or double radial immunodiffusion. It is of great importance as an aid in the diagnosis of B cell malignancies such a multiple myeloma and non-Hodgkin lymphoma, and in monitoring their therapy. In immunoelectrophoresis use 1.2 μl of sample in a well with a diameter of 1 mm against 60 μl antiserum in a 20 mm long channel. Electrophoresis for 1 hour, 20 mA. The detection limit of this technique in serum is about 25 mg/l; in the case 100 times concentrated urine is used the detection limit can be brought down to 0.5 mg/l. The fact that in about 40% of the case the Bence Jones protein was only detectable in either serum or urine emphasizes the necessity to investigate both fluids.
Concentration	25 mg/ml
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Buffer System: PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added. State: Lyophilised hyperimmune Ig fraction Serum Reconstitution: Restore with 1 ml sterile distilled water
Specificity	Specificity: The reactivity of the antiserum is directed to the hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is only reacting with Bence Jones proteins as well as free light chains of the lambda type. No reaction is obtained with intact polyclonal or monoclonal immunoglobulins. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Species: Human.

Accessory Products

Recommended Secondary Antibodies for Human Lambda Light Chain (9 products)

Catalog No.	Host	Pres.	Applications
R1317AP	Goat IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1317B	Goat IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1317F	Goat IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1317HRP	Goat IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1317T	Goat IgG (H+L chain) antibody
	Rabbit	TRITC	E, ICC/IF
				OriGene Technologies GmbH
R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1435C2	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy2
				OriGene Technologies GmbH
R1435C3	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy3
				OriGene Technologies GmbH
R1435C5	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy5	F, ICC/IF
				OriGene Technologies GmbH