AP31534FC-N Human Lambda Light Chain (free and bound) antibody

See related secondary antibodies

Search for all "Human Lambda Light Chain"

1 ml / €280.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Human Human Lambda Light Chain

AP31534FC-N

Product Description for Human Lambda Light Chain

Goat anti Human Human Lambda Light Chain.
Properties: (free and bound)
Presentation: FITC
Product is tested for Frozen Sections, Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for Human Lambda Light Chain

Product Category Primary Antibodies
Quantity 1 ml
Presentation FITC
Reactivity Hu
Applications C, Dot, E, ICC/IF, ID, IP
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
A pool of purified Bence Jones lambda proteins isolated from human urine.
Freund’s complete adjuvant is used in the first step of the immunization procedure.
Property free and bound
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins.
Application This conjugate is intended for use in direct Immunofluorescence for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the lambda type as well as free light chains or Bence Jones proteins of the lambda type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: Usually between 1/10 and 1/30.
Concentration 10.0 mg/ml
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Fluorescein isothiocyanate isomer 1
FITC
Absorption emission:
492nm / 515nm
Molar radio:
2.5
Purification:
DEAE-column Chromatography
Buffer System:
PBS, pH 7.2
No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
State:
Lyophilized purified Hyperimmune IgG fraction
Reconstitution:
Restore with 1 ml sterile distilled water.
Specificity
Specificity:
The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of human serum or plasma.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail.
Species:
Human.

Accessory Products

  • LinkedIn