Human Lambda Light Chain (free and bound) - AP31534TC-N

Product Description for Human Lambda Light Chain

Goat anti Human Human Lambda Light Chain.
Properties: (free and bound)
Presentation: TRITC
Product is tested for Frozen Sections, Dot blot, Enzyme Immunoassay, Immunodiffusion, Immunocytochemistry/Immunofluorescence, Immunoprecipitation.

Properties for Human Lambda Light Chain

Product Category	Primary Antibodies
Quantity	1 ml
Presentation	TRITC
Reactivity	Hu
Applications	C, Dot, E, ICC/IF, ID, IP
Clonality	Polyclonal
Host	Goat
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Immunogen: A pool of purified Bence Jones lambda proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Property	free and bound
Add. information	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other human serum proteins.
Application	This conjugate is intended for use in direct immunofluorescence for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the lambda type as well as free light chains or Bence Jones proteins of the lambda type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/10 and 1/40.
Concentration	10 mg/ml
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Label: Tetramethylrhodamine isothiocyanate isomer R. Fluorescent marker: To avoid nonspecific background staining, specially synthesized and exceptionally pure crystalline isomer R has been used instead of the usual racemic mixture. Although its fluorescence efficiency is less than of FITC, conjugates have the advantage of significantly less photo bleaching. This facilitates their use in quantitative cell-counting procedures.Conjugation procedure: A proprietary technique for the binding to is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life TRITC Absorption emission: 554 nm / 573 nm Molar radio: 1,9 Purification: DEAE-column Chromatography Buffer System: PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added. State: Lyophilised hyperimmune Ig fraction Reconstitution: Restore with 1 ml sterile distilled water.
Specificity	Specificity: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of human serum or plasma. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Species: Human.

Accessory Products

Recommended Secondary Antibodies for Human Lambda Light Chain (9 products)

Catalog No.	Host	Pres.	Applications
R1317AP	Goat IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1317B	Goat IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1317F	Goat IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1317HRP	Goat IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1317T	Goat IgG (H+L chain) antibody
	Rabbit	TRITC	E, ICC/IF
				OriGene Technologies GmbH
R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1435C2	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy2
				OriGene Technologies GmbH
R1435C3	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy3
				OriGene Technologies GmbH
R1435C5	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy5	F, ICC/IF
				OriGene Technologies GmbH