Complement C3c - AP31546FC-N | acris-antibodies.com

Product Description for Complement C3c

Goat anti Rabbit Complement C3c.
Presentation: FITC
Product is tested for Immunodiffusion, Immunoprecipitation.

Properties for Complement C3c

Product Category	Primary Antibodies
Quantity	1 ml
Synonyms	Complement component 3c
Presentation	FITC
Reactivity	Rb
Applications	ID, IP
Clonality	Polyclonal
Host	Goat
Isotype	IgG
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P12247 Immunogen: C3c is the major fragment resulting from C3 cleavage by C3 convertase and factor I. It is composed of an intact beta chain bound to two fragments of the alpha chain. C3c is isolated and purified from pooled normal rabbit serum. Freund’s complete adjuvant is used in the first step of the immunization procedure. GeneID: 100009121
Add. information	Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies cross-reacting with other with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application	The fluorescent immunoconjugate to rabbit C3c is used to determine the presence and pattern of C3 in tissue lesions using immunohistochemical staining techniques. Locally deposited immune complexes in tissue usually contain complement, pointing to activation of the classical pathway. Complement activation in vivo implies active disease and may contribute to the elicitation of the pathogenesis and he extent of tissue destruction. Sometimes the diagnosis can be based on directly on laboratory findings. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1/20 and 1/80.
Concentration	15,5 mg/ml
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Label: Fluorescein isothiocyanate isomer 1 FITC Absorption emission: 492nm / 515nm Molar radio: 1,2 Buffer System: PBS, pH 7.2 No preservative added, as it may interfere with the antibody activity. No foreign proteins added. State: Lyophilised hyperimmune Ig fraction Reconstitution: Restore with 1 ml sterile distilled water
Specificity	Specificity: In immunoelectrophoresis against fresh rabbit serum, a single precipitin line is obtained in the beta-1 region representing native C3. Against serum containing partly activated C3, a precipitin line is obtained which extends from the beta-1 into the alpha-2 region, demonstrating a gradient. In old serum containing totally activated C3 a single precipitin line in the alpha-2 region is obtained. Antisera to C3c cab also react with the fragments C3b, C3bi and smaller fragments, since they all carry antigenic determinants of the C3c domain. The product does not react with any other proteins component of rabbit serum or plasma. Cross-reactivity: The antiserum does not cross-react with any other component of rabbit plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail. Species: Rabbit.

Accessory Products

Recommended Secondary Antibodies for Complement C3c (9 products)

Catalog No.	Host	Pres.	Applications
R1317AP	Goat IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1317B	Goat IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1317F	Goat IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1317HRP	Goat IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1317T	Goat IgG (H+L chain) antibody
	Rabbit	TRITC	E, ICC/IF
				OriGene Technologies GmbH
R1416R	Goat IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1435C2	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy2
				OriGene Technologies GmbH
R1435C3	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy3
				OriGene Technologies GmbH
R1435C5	Goat IgG (H+L chain), adsorbed antibody
	Donkey	Cy5	F, ICC/IF
				OriGene Technologies GmbH