AP31547BT-N Albumin antibody

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1 ml / €280.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Goat anti Rat Albumin

AP31547BT-N

Product Description for Albumin

Goat anti Rat Albumin.
Presentation: Biotin
Product is tested for Frozen Sections, Enzyme Immunoassay, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western blot / Immunoblot.

Properties for Albumin

Product Category Primary Antibodies
Quantity 1 ml
Synonyms ALB, BSA, HSA, Serum Albumin
Presentation Biotin
Reactivity Rt
Applications C, E, ICC/IF, IP, R, WB
Clonality Polyclonal
Host Goat
Isotype IgG
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Polyclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P02770
Immunogen:
Albumin is a stable small polypeptide with a strong antigenicity. Its molecular weight is about 69,000. It has a high mobility in electrophoresis, shows macro-heterogeneity especially under pathological conditions and it can bind a large number of physiological and non-physiological molecules. Albumin is isolated from rat serum by sequential precipitation and purified by ion exchange chromatography and affinity chromatography. Freund’s complete adjuvant is used in the first step of the immunization procedure.
GeneID:
24186
Add. information Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Application Can be used in immunocytochemical and immunohistochemical use for the detection of albumin at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; in non-isotopic assay methodology (e.g. ELISA) to measure albumin in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays between 1/5000 and 1/15000.
Concentration 10 mg/ml
Storage Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one week
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Label:
Biotin
Molar radio:
6,4
Purification:
DEAE-column Chromatography
Buffer System:
PBS, pH 7.2
No preservative added, as it may interfere with the antibody activity. No foreign proteins added.
State:
Lyophilised hyperimmune Ig fraction
Reconstitution:
Restore with 1 ml sterile distilled water
Specificity
Specificity:
The defined antibody specificity is directed to albumin as tested against rat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin.
Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested in double radial immunodiffusion with the following results:
± rabbit
- bovine, chicken, goat, sheep
+ dog, horse, human, monkey, swine
++ guinea pig, mouse
Species:
Rat.
Gene ID 24186

Accessory Products

Proteins and/or Positive Controls

Proteins for Albumin (2 products)

Catalog No. Species Pres. Purity   Source  

Albumin

Albumin Rat
  Abnova Taiwan Corp.

Albumin (WB+ control)

Albumin Purified
  Alpha Diagnostic Intl. Inc.
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