MYLPF (1-169, His-tag) - AR39037PU-L

Product Description for MYLPF

MYLPF.
Presentation: Purified

Product Category	Proteins & Growth Factors
Quantity	0.25 mg
Synonyms	Fast skeletal myosin light chain 2, MLC2B, Myosin regulatory light chain 2, skeletal muscle
Presentation	Purified
Molecular weight	21.2 kDa
Source	E. coli
Species (Protein)	Human
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Proteins (de)

Immunogen	Swiss Prot Num: Q96A32
Purity	> 85 %
Background	Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by myosin light chain kinase (MLCK). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the actin-activated myosin ATPase activity, thereby regulating the contractile activity. MYLPF is critically important for fast and slow skeletal muscle development.
Concentration	0.25 mg/ml (determined by Bradford assay)
General Readings	Wang Y, et al. (2007) FASEB J. 21(9):2205-14. Gittings W, et al. (2011) J Muscle Res Cell Motil. 31(5-6):337-48.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Description	Description: Recombinant human MYLPF protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. AA Sequence: `MGSSHHHHHH SSGLVPRGSH MAPKRAKRRT VEGGSSSVFS MFDQTQIQEF KEAFTVIDQN RDGIIDKEDL RDTFAAMGRL NVKNEELDAM MKEASGPINF TVFLTMFGEK LKGADPEDVI TGAFKVLDPE GKGTIKKKFL EELLTTQCDR FSQEEIKNMW AAFPPDVGGN VDYKNICYVI THGDAKDQE` Molecular weight: (189aa), confirmed by MALDI-TOF
Format	Buffer System: 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl Purity: >85% State: Liquid purified protein Purified