Product Description for AKR1C4
AKR1C4.
Presentation: Purified
Presentation: Purified
Properties for AKR1C4
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 0.1 mg |
| Synonyms | 3-alpha-HSD1, 3-alpha-hydroxysteroid dehydrogenase type I, Aldo-keto reductase family 1 member C4, CDR, CHDR, Chlordecone reductase, DD-4, DD4, Dihydrodiol dehydrogenase 4, HAKRA |
| Presentation | Purified |
| Molecular weight | 39.2 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
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| Purity | > 90 % |
| Background | AKR1C4 belongs to aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. It catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. |
| Concentration | 1.0 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay 1.Prepare a 1 ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2.Add 50ul of recombinant AKR1C4 solution with various concentrations (1ug, 2ug) in 750ul reaction buffer. 3.Mix by inversion and incubate at 25°C for 2.5 minutes. 4.Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5.Record the increase at A340nm for 3 minutes. |
| General Readings | Stayrook KR. et al. (2008) Mol Pharmacol. 73:607-612. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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