Product Description for Adenosine deaminase
Adenosine deaminase.
Presentation: Purified
Presentation: Purified
Properties for Adenosine deaminase
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 50 µg |
| Synonyms | ADA, Adenosine aminohydrolase |
| Presentation | Purified |
| Molecular weight | 42.9 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
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| Purity | > 85 % |
| Background | ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay 1.Prepare a 1.5 ml reaction mix: the final concentrations are 53.3mM potassium phosphate, 0.045mM adenosine, 0.003% (w/v) bovine serum. 2.Add recombinant ADA protein with various concentrations (0.1ug, 0.2) in assay buffer. 3.Mix by inversion and record A260nm for approximately 5 minutes. |
| General Readings | Gines S., et al. (2002) Biochem. 361:203-209. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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