AR39072PU-N Pyrophosphatase 1 / PPA1 (1-176, His-tag)

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0.1 mg / €320.00
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Pyrophosphatase 1 / PPA1

AR39072PU-N

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  • AR39072PU-N

Product Description for Pyrophosphatase 1 / PPA1

Pyrophosphatase 1 / PPA1.
Presentation: Purified

Properties for Pyrophosphatase 1 / PPA1

Product Category Proteins & Growth Factors
Quantity 0.1 mg
Synonyms IOPPP, Inorganic pyrophosphatase, PP, PPase, Pyrophosphate phospho-hydrolase
Presentation Purified
Molecular weight 21.9 kDa
Source E. coli
Species (Protein) E. coli
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Proteins (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P0A7A9
Purity > 95 %
Background Inorganic pyrophosphatase (PPA1, E.coli ppa) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations.
Concentration 1.0 mg/ml (determined by Bradford assay)
General Readings Carman GM., et al (2006) Trends Biochem. Sci. 31 (12): 694–9.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Description
Description:
Recombinant E.coli ppa protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE AAKAEIVASF ERAKNK
Molecular weight:
(196aa), confirmed by MALDI-TOF
Format
Buffer System:
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
Purity:
>95%
State:
Liquid purified protein
Purified
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