Product Description for Protein phosphatase 1G / PPM1G
Protein phosphatase 1G / PPM1G.
Presentation: Purified
Presentation: Purified
Properties for Protein phosphatase 1G / PPM1G
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 50 µg |
| Synonyms | PP2C-gamma, PPM1C, Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma |
| Presentation | Purified |
| Molecular weight | 61.4 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 85 % pure by SDS - PAGE |
| Background | PPM1G, as known as protein phosphatase 1G, is a member of the PP2C family of Ser/Thr protein phosphatases that is negative regulators of cell stress response pathways. This enzyme localizes to the cytoplasm and is widely expressed, with most abundant expression detected in testis, skeletal muscle and heart. It is necessary for the dephosphorylation of pre-mRNA splicing factors, which is an important process for the formation of the functional spliceosome. |
| Concentration | 1.0 mg/ml (determined by BCA assay) |
| Protocols | Activity Assay 1.Prepare a 200ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP. 2.Pre-warm reaction mix in heat block at 37°C 3.Add recombinant PPM1G solution with various concentrations (2ug, 3ug, 4ug) in 200 ul reaction buffer. 4.Mix by inversion and incubate at 37°C for 10 minutes. 5.Stop the reaction by adding the 800 ul of 0.1N NaOH. 6.Record the absorbance at 405nm |
| General Readings |
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| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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