Product Description for PPP1CA
PPP1CA.
Presentation: Purified
Presentation: Purified
Properties for PPP1CA
| Product Category | Proteins & Growth Factors |
|---|---|
| Quantity | 50 µg |
| Synonyms | PP-1A, PPP1A, Serine/threonine-protein phosphatase PP1-alpha catalytic subunit |
| Presentation | Purified |
| Molecular weight | 39.7 kDa |
| Source | E. coli |
| Species (Protein) | Human |
| Shipping to | Worldwide |
| PDF datasheet | View Datasheet |
| Manufacturer | OriGene Technologies GmbH |
| Material safety datasheet | MSDS for Proteins (de) |
Datasheet Extract
| Immunogen |
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|---|---|
| Purity | > 80 % |
| Background | PPP1CA, also known as serine/threonine-protein phosphatase PP1-alpha catalytic subunit, is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. This protein may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II. |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Protocols | Activity Assay 1.Prepare a 200 ul reaction mix into a suitable container : The final concentrations are 10mM Tris (pH 7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP. 2.Pre-warm reaction mix in heat block at 37°C. 3.Add recombinant PPP1CA solution with various concentrations (2ug, 3ug) in 200ul reaction buffer. 4.Mix by inversion and incubate at 37°C for 10 minutes. 5.Stop the reaction by adding the 800ul of 0.1N NaOH. 6.Record the absorbance at 405nm. |
| General Readings | Trinkle-Mulcahy L., et al. (2001) J. Cell Sci. 114:4219-4228. |
| Storage | Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
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