BM370 Desmoglein 1 + 2 antibody

Swiss Prot Num:

Q14126

Immunogen:

"Band 3" polypeptide of isolated desmosomes from Bovine muzzle epidermis

GeneID:

1829

1. Koch PJ, Walsh MJ, Schmelz M, Goldschmidt MD, Zimbelmann R, Franke WW. Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur J Cell Biol. 1990 Oct;53(1):1-12. PubMed PMID: 1706270.
2. Schmelz M, Duden R, Cowin P, Franke WW. A constitutive transmembrane glycoprotein of Mr 165,000 (desmoglein) in epidermal and non-epidermal desmosomes. II. Immunolocalization and microinjection studies. Eur J Cell Biol. 1986 Dec;42(2):184-99. PubMed PMID: 2434328.
3. Wheeler GN, Parker AE, Thomas CL, Ataliotis P, Poynter D, Arnemann J, et al. Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules. Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):4796-800. PubMed PMID: 1711210. (Free PMC Article available)
4. Schmelz M, Franke WW. Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes. Eur J Cell Biol. 1993 Aug;61(2):274-89. PubMed PMID: 8223718.
5. Schäfer S, Koch PJ, Franke WW. Identification of the ubiquitous human desmoglein, Dsg2, and the expression catalogue of the desmoglein subfamily of desmosomal cadherins. Exp Cell Res. 1994 Apr;211(2):391-9. PubMed PMID: 8143788.
6. Schäfer S, Stumpp S, Franke WW. Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues. Differentiation. 1996 May;60(2):99-108. PubMed PMID: 8641550.
7. Hatzfeld M, Haffner C, Schulze K, Vinzens U. The function of plakophilin 1 in desmosome assembly and actin filament organization. J Cell Biol. 2000 Apr 3;149(1):209-22. PubMed PMID: 10747098. (Free PMC Article available, 12 images available)
8. Caldelari R, de Bruin A, Baumann D, Suter MM, Bierkamp C, Balmer V, et al. A central role for the armadillo protein plakoglobin in the autoimmune disease pemphigus vulgaris. J Cell Biol. 2001 May 14;153(4):823-34. PubMed PMID: 11352942. (Free PMC Article available, 8 images available)
9. Akat K, Mennel HD, Kremer P, Gassler N, Bleck CK, Kartenbeck J. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 2003 Oct;106(4):337-47. Epub 2003 Jul 5. PubMed PMID: 12845453.
10. Kartenbeck J, Haselmann U, Gassler N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur J Cell Biol. 2005 Mar;84(2-3):417-30. PubMed PMID: 15819418.
11. Bosch FX, Andl C, Abel U, Kartenbeck J. E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: a comparison of E-cadherin with desmosomal components. Int J Cancer. 2005 May 1;114(5):779-90. PubMed PMID: 15609307.

Prior to reconstitution store at 2-8°C.
Following reconstitution store undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Purification:

Affinity Chromatography on Protein A

Buffer System:

Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer

State:

Lyophilized purified Ig fraction
Purified

Reconstitution:

Restore with 1.0 ml distilled water.

Species reactivity (tested):

Human, Bovine, Rat and Mouse.

Specificity:

This antibody recognizes Desmoglein 1+2 (desmosome-specific cadherins) in desmosome-bearing cells. It does not react with cells containing plaque-bearing junctions of non-desmosomal type.
Tumors Specifically Detected: All epithelium-derived tumors, notably carcinomas, and meningiomas.
Polypeptide Reacting: Mr 165000 Desmoglein, "band 3" polypeptide of the desmosomal complex.
Epitope Recognized: Localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.
Tested Reactivities on Cultured Cell Lines: A-431, MCF-7, TR-146, PLC and various other Human epithelial cell lines; Bovine BMGE+H, BMGE-H, MDBK.