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BM5014 Adeno-Associated Virus 2 / AAV2 (VP1 / VP2) antibody

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50 µg / €380.00
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Quick Overview

Mouse anti Adeno-Associated Virus 2 / AAV2 A69

BM5014

Product Description for Adeno-Associated Virus 2 / AAV2

Mouse anti Adeno-Associated Virus 2 / AAV2 A69.
Properties: (VP1 / VP2)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Adeno-Associated Virus 2 / AAV2

Product Category Primary Antibodies
Quantity 50 µg
Synonyms AAV-2
Presentation Purified
Applications C, ICC/IF, IP, WB
Clonality Monoclonal
Clone A69
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Adeno-associated virus capsid proteins and virus particles.
Property VP1 / VP2
Isotype control AM03095PU-N
Application Western Blot.
Immunofluorescence Microscopy.
Immunoprecipitation.
Immunohistochemistry on Frozen Sections: 1/10.
Incubation Time: 1h at RT.
Dilution buffer: PBS or Tris-buffered saline.
General Readings
  1. Wistuba A, Weger S, Kern A, Kleinschmidt JA. Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins. J Virol. 1995 Sep;69(9):5311-9. PubMed PMID: 7636974. (Free PMC Article available)
  2. Wistuba A, Kern A, Weger S, Grimm D, Kleinschmidt JA. Subcellular compartmentalization of adeno-associated virus type 2 assembly. J Virol. 1997 Feb;71(2):1341-52. PubMed PMID: 8995658. (Free PMC Article available)
  3. Wobus CE, Hügle-Dörr B, Girod A, Petersen G, Hallek M, Kleinschmidt JA. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J Virol. 2000 Oct;74(19):9281-93. PubMed PMID: 10982375. (Free PMC Article available, 6 images available)
Storage Store the antibody at 2-8°C after reconstitution.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
PBS
Preservatives:
0.09% Sodium Azide
Stabilizers:
0.5% BSA
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore with 1 ml distilled Water
Specificity
Specificity:
Recognized AAV-2, found in Human and Monkey.
A69 reacts with VP1 and VP2 of adeno-associated virus which are highly enriched in the nucleus. Epitope mapping experiments (Wobus et al., see references) identified aa169 to aa184 of VP2 and (with reduced intensity) aa123 to aa136 of VP1 capsid proteins as the specific binding region.
Weak cross-reaction with serotypes 1, 3, and 6.

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