Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Adeno-associated Virus / AAV

BM5015 Adeno-associated Virus / AAV (VP1 + VP2 + VP3) antibody

See related secondary antibodies

Search for all "Adeno-associated Virus / AAV"

50 µg / €400.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

View Datasheet

Quick Overview

Mouse anti Human, Monkey Adeno-associated Virus / AAV B1

BM5015

Product Description for Adeno-associated Virus / AAV

Mouse anti Human, Monkey Adeno-associated Virus / AAV B1.
Properties: (VP1 + VP2 + VP3)
Presentation: Purified
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Adeno-associated Virus / AAV

Product Category Primary Antibodies
Quantity 50 µg
Synonyms AAV1, AAV2, AAV3, AAV4, AAV5
Presentation Purified
Reactivity Hu, Mky
Applications C, ICC/IF, IP, WB
Clonality Monoclonal
Clone B1
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Immunogen:
Adeno-associated virus 2 capsid proteins and virus particles.
Property VP1 + VP2 + VP3
Isotype control AM03095PU-N, SM10P (for use in human samples)
Application Immunohistochemistry on Frozen Sections: 1/10.
Immunohistochemistry on Paraffin Sections: 1/10 (microwave treatment recommended).
Western Blot: 1/250.
(Dilute with PBS or TBS).
Immunoprecipitation (mainly free VP proteins).
Affinity Chromatography.
Immunofluorescence Microscopy.
General Readings
  1. Wistuba A, Weger S, Kern A, Kleinschmidt JA. Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins. J Virol. 1995 Sep;69(9):5311-9. PubMed PMID: 7636974. (Free PMC Article available)
  2. Wistuba A, Kern A, Weger S, Grimm D, Kleinschmidt JA. Subcellular compartmentalization of adeno-associated virus type 2 assembly. J Virol. 1997 Feb;71(2):1341-52. PubMed PMID: 8995658. (Free PMC Article available)
  3. Wobus CE, Hügle-Dörr B, Girod A, Petersen G, Hallek M, Kleinschmidt JA. Monoclonal antibodies against the adeno-associated virus type 2 (AAV-2) capsid: epitope mapping and identification of capsid domains involved in AAV-2-cell interaction and neutralization of AAV-2 infection. J Virol. 2000 Oct;74(19):9281-93. PubMed PMID: 10982375. (Free PMC Article available, 6 images available)
  4. Sato Y, Asahi Y, Iwasaki T, Matsukura T, Kurata T, Sata T. Detection of adeno-associated virus type 2 in patients with viral infection. Jpn J Infect Dis. 1999 Apr;52(2):50-1. PubMed PMID: 10816616.
  5. Zhang HG, Xie J, Dmitriev I, Kashentseva E, Curiel DT, Hsu HC, et al. Addition of six-His-tagged peptide to the C terminus of adeno-associated virus VP3 does not affect viral tropism or production. J Virol. 2002 Dec;76(23):12023-31. PubMed PMID: 12414944. (Free PMC Article available, 5 images available)
  6. Yan Z, Zak R, Luxton GW, Ritchie TC, Bantel-Schaal U, Engelhardt JF. Ubiquitination of both adeno-associated virus type 2 and 5 capsid proteins affects the transduction efficiency of recombinant vectors. J Virol. 2002 Mar;76(5):2043-53. PubMed PMID: 11836382. (Free PMC Article available, 4 images available)
  7. Sonntag F, Köther K, Schmidt K, Weghofer M, Raupp C, Nieto K, et al. The assembly-activating protein promotes capsid assembly of different adeno-associated virus serotypes. J Virol. 2011 Dec;85(23):12686-97. doi: 10.1128/JVI.05359-11. Epub 2011 Sep 14. PubMed PMID: 21917944. (Free PMC Article available, 10 images available)
  8. Naumer M, Sonntag F, Schmidt K, Nieto K, Panke C, Davey NE, et al. Properties of the adeno-associated virus assembly-activating protein. J Virol. 2012 Dec;86(23):13038-48. doi: 10.1128/JVI.01675-12. Epub 2012 Sep 26. PubMed PMID: 23015698. (Free PMC Article available, 8 images available)
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A (≥ 95% pure by SDS-PAGE)
Buffer System:
PBS, pH 7.4
Preservatives:
0.09% Sodium Azide
Stabilizers:
0.5% BSA
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore with 1 ml sterile PBS.
Species Reactivity
Species reactivity (tested):
Human and Monkey.
Specificity
Specificity:
Useful for Immunolocalization studies of capsid formation in combination with monoclonal antibody A20 (Cat. No. BM5010).
B1 reacts with free VP 1, VP 2 and VP 3 of adeno-associated virus and at a very reduced degree with assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm.
Epitope mapping experiments (Wobus et al., see below) identified amino acids 726 to aa733 (C-terminus; common to all 3 VP proteins) as the specific binding region.
The antibody is also useful for characterization of different stages of infection.
Reacts with AAV-2, found in Human and Monkey, and all published AAV serotypes (AAV-4 only weak cross-reaction, 11 &12 no cross-reaction).

Accessory Products

Recommended Secondary Antibodies for Adeno-associated Virus / AAV (11 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
    OriGene Technologies GmbH
Click here to see all secondary antibodies for Adeno-associated Virus / AAV
Compare Products

You have no items to compare.

My Cart

You have no items in your shopping cart.

Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn