Chromogranin A - BM5082 | acris-antibodies.com

Product Description for Chromogranin A

Mouse anti Human, Monkey, Porcine, Rabbit, Rat Chromogranin A LK2H10.
Presentation: Ascites
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot, Paraffin Sections.

Properties for Chromogranin A

Product Category	Primary Antibodies
Quantity	1 ml
Synonyms	CHGA, CgA, Chromogranin-A, Pituitary secretory protein I, SP-I
Presentation	Ascites
Reactivity	Hu, Mky, Por, Rb, Rt
Applications	C, ICC/IF, P, WB
Clonality	Monoclonal
Clone	LK2H10
Host	Mouse
Isotype	IgG1
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P10645 Immunogen: Isolated from Human pheochromocytoma. GeneID: 1113
Application	Immunoblotting. Immunofluorescence. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/10 (In No case proteolytic pretreatment). Recommended Positive Control: Pancreas, adrenal medulla. *Dilution Buffer:* Dilute immediately before use with TBS.
Background	Neurons and neuroendocrine cells contain membrane-delimited pools of peptide hormones, biogenic amines, and neurotransmitters with a characteristic electron-dense appearance. These vesicles, which are present throughout the neuroendocrine system and in a variety of neurons, store and release chromogranins and secretogranins, a unique group of acidic, soluble secretory proteins. Chromogranin A, a protein of 439 amino acids which is encoded on chromosome 14 is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
General Readings	Lloyd RV, Wilson BS. Specific endocrine tissue marker defined by a monoclonal antibody. Science. 1983 Nov 11;222(4624):628-30. PubMed PMID: 6635661. Taupenot L, Harper KL, O'Connor DT. The chromogranin-secretogranin family. N Engl J Med. 2003 Mar 20;348(12):1134-49. PubMed PMID: 12646671. Wilson BS, Lloyd RV. Detection of chromogranin in neuroendocrine cells with a monoclonal antibody. Am J Pathol. 1984 Jun;115(3):458-68. PubMed PMID: 6375394. (Free PMC Article available)
Storage	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Preservatives: 0.09% Sodium Azide State: Liquid Ascites Ascites
Species Reactivity	Species reactivity (tested): Human, Monkey, Rat, Rabbit, Pig.
Specificity	Specificity: This Monoclonal antibody LK2H10 represents an excellent marker for neurohomone peptide producing cells (APUD) and for most neuroendocrine, neuronal and adrenal tumors: e.g. pheochromocytoma, paraganglioma, neuroblastoma, pancreatic islet cell carcinoma, gastroadenomas from pituitary and parathyroid gland. Weak reaction with poorly granulated endocrine cells, Merkel cell tumors of skin and small cell lung carcinoma. The antibody reacts with the 68 kD protein (Chromogranin A). The antigen is localized in secretory granules and, therefore, poorly granulated cells react weakly or not at all.
Gene ID	1113

Accessory Products

Proteins and/or Positive Controls

Proteins for Chromogranin A (12 products)

Catalog No.	Species	Pres.	Purity	Source
AR09190PU-L	CHGA (Vasostatin-2 chain) (19-131)
	Human	Purified	> 95 % by SDS - PAGE	E. coli	0.5 mg / €820.00
					OriGene Technologies GmbH
AR09190PU-N	CHGA (Vasostatin-2 chain) (19-131)
	Human	Purified	> 95 % by SDS - PAGE	E. coli	0.1 mg / €320.00
					OriGene Technologies GmbH
TP300492	Chromogranin A
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €748.00
					OriGene Technologies, Inc.
AR09204PU-L	Chromogranin A (19-457, His-tag)
	Human	Purified	> 80 % by SDS - PAGE	E. coli	0.25 mg / €1,070.00
					OriGene Technologies GmbH
AR09204PU-N	Chromogranin A (19-457, His-tag)
	Human	Purified	> 80 % by SDS - PAGE	E. coli	50 µg / €400.00
					OriGene Technologies GmbH
H00001113-P01	Chromogranin A
	Human
					Abnova Taiwan Corp.
H00001113-P01-10	Chromogranin A
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue
					Abnova Taiwan Corp.
H00001113-P01-25	Chromogranin A
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue
					Abnova Taiwan Corp.
H00001113-Q01-10	Chromogranin A
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00001113-Q01-25	Chromogranin A
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
NBC1-26369	Chromogranin A
			Protein
					Novus Biologicals Inc.
NBC1-27996	Chromogranin A
			Protein
					Novus Biologicals Inc.

Positive controls for Chromogranin A (2 products)

Catalog No.	Species
H00001113-T01	CHGA 293T Cell Transient Overexpression Lysate(Denatured)

		Abnova Taiwan Corp.
NBL1-09149	Chromogranin A Lysate

		Novus Biologicals Inc.

Recommended Secondary Antibodies for Chromogranin A (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

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BM5082 Chromogranin A antibody

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