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BM5098 Cingulin antibody

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5 ml / €300.00
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Quick Overview

Mouse anti Bovine, Human, Mouse, Xenopus Cingulin 139.3.4

BM5098

Product Description for Cingulin

Mouse anti Bovine, Human, Mouse, Xenopus Cingulin 139.3.4.
Presentation: Supernatant
Product is tested for Frozen Sections, Western blot / Immunoblot, Paraffin Sections.

Properties for Cingulin

Product Category Primary Antibodies
Quantity 5 ml
Synonyms CGN, KIAA1319
Presentation Supernatant
Reactivity Bov, Hu, Ms, Xen
Applications C, P, WB
Clonality Monoclonal
Clone 139.3.4
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q9P2M7
Immunogen:
Synthetic peptide (aa. 4-24)
GeneID:
57530
Application Immunoblotting.
Immunohistochemistry on Frozen and Paraffin Sections (only after microwave treatment): Ready-to-use.
On cultured cell fixation with paraformaldehyde is recommended for optimal staining.
Background Cingulin, a polypeptide of 126.5 kD (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5 (very similar to that of symplekin, another junctional protein).
General Readings
  1. Keon BH, Schäfer S, Kuhn C, Grund C, Franke WW. Symplekin, a novel type of tight junction plaque protein. J Cell Biol. 1996 Aug;134(4):1003-18. PubMed PMID: 8769423. (Free PMC Article available)
  2. Zahraoui A, Louvard D, Galli T. Tight junction, a platform for trafficking and signaling protein complexes. J Cell Biol. 2000 Nov 27;151(5):F31-6. PubMed PMID: 11086016. (Free PMC Article available, 1 images available)
  3. Langbein L, Grund C, Kuhn C, Praetzel S, Kartenbeck J, Brandner JM, et al. Tight junctions and compositionally related junctional structures in mammalian stratified epithelia and cell cultures derived therefrom. Eur J Cell Biol. 2002 Aug;81(8):419-35. PubMed PMID: 12234014.
  4. Langbein L, Pape UF, Grund C, Kuhn C, Praetzel S, Moll I, et al. Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas. Eur J Cell Biol. 2003 Aug;82(8):385-400. PubMed PMID: 14533737.
Storage Store the antibody undiluted at 2-8°C.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Liquid Culture Supernatant
Supernatant
Species Reactivity
Species reactivity (tested):
Human, Bovine, Mouse and Xenopus laevis.Negative with Rabbit.
Specificity
Specificity:
This monoclonal antibody reacts specifically with Cingulin (a 150 kD polypeptide) present in tight junctions (zonula occludens) of polar epithelia (e.g. colon, duodenum, kidney, liver, pancreas), of transitional epithelia (e.g. bladder epithelium), and of stratified epithelia (e.g. skin, foot sole, tongue, oesophagus, vagina).
Negative with endothelia of vessels and capillaries (e.g. in heart and brain).
In contrast to Symplekin, using Monoclonal antibody 139.4 Cingulin has been localized exclusively to the tight junction complex.
Tested Reactivities on Cultured Cell Lines: Carcinoma cell lines (e.g. MCF-7, Caco-2, PLC, A-431), SV80 (fibroblasts), Glioma; MDBK, MDCK, BMGE.
Gene ID 57530

Accessory Products

Proteins and/or Positive Controls

Positive controls for Cingulin (1 products)

Catalog No. Species Pres. Purity   Source  

CGN overexpression lysate
CGN overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

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Mouse IgM (Mu chain specific), adsorbed antibody
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Mouse IgM (Mu chain specific), adsorbed antibody
Mouse IgM (Mu chain specific), adsorbed antibody Goat FITC Ms
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Mouse IgM (Mu chain specific), adsorbed antibody
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Mouse IgM (Mu chain specific), adsorbed antibody
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