Switch to Europe/Worldwide website Switch to German website Switch to French website Switch to OriGene Technologies, Inc. (North America)
Home » Junction plakoglobin

BM5100 Junction plakoglobin antibody

See related secondary antibodies

Search for all "Junction plakoglobin"

50 µg / €310.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

View Datasheet

Published recently!

Quick Overview

Mouse anti Bovine, Chicken, Human, Mouse, Rat, Zebrafish Junction plakoglobin PG 5.1

BM5100

Product Description for Junction plakoglobin

Mouse anti Bovine, Chicken, Human, Mouse, Rat, Zebrafish Junction plakoglobin PG 5.1.
Presentation: Purified
Product is tested for Paraffin Sections, Frozen Sections, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western blot / Immunoblot.

Properties for Junction plakoglobin

Product Category Primary Antibodies
Quantity 50 µg
Synonyms CTNNG, Catenin gamma, DP3, Desmoplakin III, Desmoplakin-3, JUP
Presentation Purified
Reactivity Bov, Chk, Hu, Ms, Rt, Ze
Applications C, ICC/IF, IP, P, WB
Clonality Monoclonal
Clone PG 5.1
Host Mouse
Isotype IgG2b
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P14923
Immunogen:
Plakoglobin, "band 5" protein from Bovine snout epidermis
GeneID:
3728
Isotype control AM03110PU-N, SM12P
Application Immunoblotting. 
Immunoprecipitation. 
Immunofluorescence Microscopy: 1/5 (with PBS, pH 7.4). 
Immunohistochemistry on Frozen Sections. 
Incubation Time: 1 h at RT.
Immunohistochemistry on Paraffin Sections has been described (See reference product citation 1, Brodehl, et al, 2013 ).
Background Gamma Catenin is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of gamma Catenin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. It is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.
Product Citations
Purchased from Acris:
  1. Brodehl A, Dieding M, Klauke B, Dec E, Madaan S, Huang T, et al. The novel desmin mutant p.A120D impairs filament formation, prevents intercalated disk localization, and causes sudden cardiac death. Circ Cardiovasc Genet. 2013 Dec;6(6):615-23. doi: 10.1161/CIRCGENETICS.113.000103. Epub 2013 Nov 7. PubMed PMID: 24200904.
General Readings
  1. Cowin P, Kapprell HP, Franke WW, Tamkun J, Hynes RO. Plakoglobin: a protein common to different kinds of intercellular adhering junctions. Cell. 1986 Sep 26;46(7):1063-73. PubMed PMID: 3530498.
  2. Koch PJ, Walsh MJ, Schmelz M, Goldschmidt MD, Zimbelmann R, Franke WW. Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules. Eur J Cell Biol. 1990 Oct;53(1):1-12. PubMed PMID: 1706270.
  3. Wahl JK, Sacco PA, McGranahan-Sadler TM, Sauppé LM, Wheelock MJ, Johnson KR. Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J Cell Sci. 1996 May;109 ( Pt 5):1143-54. PubMed PMID: 8743961.
  4. Chitaev NA, Leube RE, Troyanovsky RB, Eshkind LG, Franke WW, Troyanovsky SM. The binding of plakoglobin to desmosomal cadherins: patterns of binding sites and topogenic potential. J Cell Biol. 1996 Apr;133(2):359-69. PubMed PMID: 8609168. (Free PMC Article available)
  5. Luft FC. Wingless armadillos and their clinical relevance. J Mol Med (Berl). 1997 Jan;75(1):1-2. PubMed PMID: 9020377.
  6. Cerdà J, Reidenbach S, Prätzel S, Franke WW. Cadherin-catenin complexes during zebrafish oogenesis: heterotypic junctions between oocytes and follicle cells. Biol Reprod. 1999 Sep;61(3):692-704. PubMed PMID: 10456847.
  7. Caldelari R, de Bruin A, Baumann D, Suter MM, Bierkamp C, Balmer V, et al. A central role for the armadillo protein plakoglobin in the autoimmune disease pemphigus vulgaris. J Cell Biol. 2001 May 14;153(4):823-34. PubMed PMID: 11352942. (Free PMC Article available, 8 images available)
  8. South AP, Wan H, Stone MG, Dopping-Hepenstal PJ, Purkis PE, Marshall JF, et al. Lack of plakophilin 1 increases keratinocyte migration and reduces desmosome stability. J Cell Sci. 2003 Aug 15;116(Pt 16):3303-14. Epub 2003 Jul 2. PubMed PMID: 12840072.
  9. Akat K, Mennel HD, Kremer P, Gassler N, Bleck CK, Kartenbeck J. Molecular characterization of desmosomes in meningiomas and arachnoidal tissue. Acta Neuropathol. 2003 Oct;106(4):337-47. Epub 2003 Jul 5. PubMed PMID: 12845453.
  10. Kartenbeck J, Haselmann U, Gassler N. Synthesis of junctional proteins in metastasizing colon cancer cells. Eur J Cell Biol. 2005 Mar;84(2-3):417-30. PubMed PMID: 15819418.
  11. Pieperhoff S, Franke WW. The area composita of adhering junctions connecting heart muscle cells of vertebrates - IV: coalescence and amalgamation of desmosomal and adhaerens junction components - late processes in mammalian heart development. Eur J Cell Biol. 2007 Jul;86(7):377-91. Epub 2007 May 29. PubMed PMID: 17532539.
  12. Boehnke K, Mirancea N, Pavesio A, Fusenig NE, Boukamp P, Stark HJ. Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents. Eur J Cell Biol. 2007 Dec;86(11-12):731-46. Epub 2007 Feb 9. PubMed PMID: 17292509.
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Purification:
Affinity Chromatography on Protein A
Buffer System:
Final solution contains PBS buffer, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
State:
Lyophilized purified IgG fraction
Purified
Reconstitution:
Restore in 1 ml distilled water
Species Reactivity
Species reactivity (tested):
Human, Bovine, Rat, Mouse, Chicken and Zebrafish.
Specificity
Specificity:
PG 5.1 represents an excellent marker for all forms of intercellular adhering junctions, such as: Desmosomes of epithelial and myocardial cells (incl. cultured cells); Zonulae and fasciae adherentes of epithelia, endothelia of blood vessels and myocardial cells; adherens-type junctions (e.g. lens tissue, pigmented retinal cells, Sertoli cells of testis). The PG 5.1 epitope maps within the C-terminus at the extreme end of repeat 13 (aa 632-687) of plakoglobin.
Polypeptide Reacting: Mr 83 000 Plakoglobin, "band 5" polypeptide of intercellular adhering junctions (identical to γ-catenin). 
Reactivity on Cultured Cell Lines: Cell cultures forming monolayers (from tested species cited below).
Gene ID 3728

Accessory Products

Proteins and/or Positive Controls

Proteins for Junction plakoglobin (3 products)

Catalog No. Species Pres. Purity   Source  

Junction plakoglobin (transcript variant 1)
Junction plakoglobin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €748.00
  OriGene Technologies, Inc.

Junction plakoglobin
Junction plakoglobin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Junction plakoglobin
Junction plakoglobin Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Junction plakoglobin (4 products)

Catalog No. Species Pres. Purity   Source  

gamma Catenin Lysate
Western Blot: gamma Catenin Lysate [NBL1-12118] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for JUP
  Novus Biologicals Inc.

JUP overexpression lysate
JUP overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

JUP overexpression lysate
JUP overexpression lysate
0.1 mg / €295.00
  OriGene Technologies, Inc.

JUP overexpression lysate
JUP overexpression lysate
0.1 mg / €495.00
  OriGene Technologies, Inc.

Recommended Secondary Antibodies for Junction plakoglobin (11 products)

Catalog No. Host Clone/Iso. Pres.   React. Applications  

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit AP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Biotin C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit FITC E, ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit HRP C, E, WB
    OriGene Technologies GmbH

Mouse IgG (H+L chain) antibody
Mouse IgG (H+L chain) antibody Rabbit Texas Red ICC/IF
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Rabbit PE F
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Goat PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody Donkey PE
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy2
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy3
    OriGene Technologies GmbH

Mouse IgG (H+L chain), adsorbed antibody
Mouse IgG (H+L chain), adsorbed antibody Goat Cy5
    OriGene Technologies GmbH
Click here to see all secondary antibodies for Junction plakoglobin
Compare Products

You have no items to compare.

My Cart

You have no items in your shopping cart.

Service
  • OriGene Technologies GmbH
  • Schillerstraße 5
  • D-32052 Herford
  • Germany
  • phone: +49-5221-34606-0
  • free call: 0800-227 47 46 (Germany only)
  • fax: +49-5221-34606-11
  • email: info-de@origene.com
  • orders: ordering-de@origene.com
 
© Copyright 1998 - 2018 OriGene Technologies GmbH
  • LinkedIn