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BM5101 Symplekin / SYMPK antibody

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5 ml / €300.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Quick Overview

Mouse anti Human, Rat Symplekin / SYMPK Sym-TJ-E150

BM5101

Product Description for Symplekin / SYMPK

Mouse anti Human, Rat Symplekin / SYMPK Sym-TJ-E150.
Presentation: Supernatant
Product is tested for Frozen Sections, Western blot / Immunoblot.

Properties for Symplekin / SYMPK

Product Category Primary Antibodies
Quantity 5 ml
Synonyms SPK
Presentation Supernatant
Reactivity Hu, Rt
Applications C, WB
Clonality Monoclonal
Clone Sym-TJ-E150
Host Mouse
Isotype IgG1
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
Q92797
Immunogen:
Human symplekin
GeneID:
8189
Application Immunoblotting.
Immunohistochemistry: For nucleoplasmic staining a modified Carnoy fixation procedure is suggested (60% ethanol, 30% chloroform, 10% acetic acid) for 20 min, followed by a 10 min ethanol wash.
Background Symplekin, 126.5 kD polypeptide (calculated from aa sequence data); apparent Mr 150 kD (after SDS-PAGE); pI 6.5
General Readings
  1. Keon BH, Schäfer S, Kuhn C, Grund C, Franke WW. Symplekin, a novel type of tight junction plaque protein. J Cell Biol. 1996 Aug;134(4):1003-18. PubMed PMID: 8769423. (Free PMC Article available)
  2. Zahraoui A, Louvard D, Galli T. Tight junction, a platform for trafficking and signaling protein complexes. J Cell Biol. 2000 Nov 27;151(5):F31-6. PubMed PMID: 11086016. (Free PMC Article available, 1 images available)
  3. Langbein L, Grund C, Kuhn C, Praetzel S, Kartenbeck J, Brandner JM, et al. Tight junctions and compositionally related junctional structures in mammalian stratified epithelia and cell cultures derived therefrom. Eur J Cell Biol. 2002 Aug;81(8):419-35. PubMed PMID: 12234014.
  4. Langbein L, Pape UF, Grund C, Kuhn C, Praetzel S, Moll I, et al. Tight junction-related structures in the absence of a lumen: occludin, claudins and tight junction plaque proteins in densely packed cell formations of stratified epithelia and squamous cell carcinomas. Eur J Cell Biol. 2003 Aug;82(8):385-400. PubMed PMID: 14533737.
Storage Store the antibody undiluted at 2-8°C.
DO NOT FREEZE!
Shelf life: one year from despatch.
Format
State:
Liquid culture supernatant
Supernatant
Specificity
Specificity:
The mab reacts specifically with symplekin (a 150 kDa polypeptide) present in 1) tight junctions (zonula occludens) of polar epithelia and Sertoli cells of testis (but not their vascular endothelia!) and 2) exclusively in the nucleoplasm of these and many other cell types which do not form tight junctions (e.g. stratified epithelia such as oesophagus, tongue mucosa, epidermis; squamous cell carcinoma; nonepithelia such as fibroblasts, endothelia, lymphocytes).
Species:
Human, Rat.
Gene ID 8189

Accessory Products

Proteins and/or Positive Controls

Proteins for Symplekin / SYMPK (3 products)

Catalog No. Species Pres. Purity   Source  

Symplekin / SYMPK
Symplekin / SYMPK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. 		HEK293 cells
20 µg / €788.00
  OriGene Technologies, Inc.

Symplekin / SYMPK
Symplekin / SYMPK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Symplekin / SYMPK
Symplekin / SYMPK Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
  Abnova Taiwan Corp.

Positive controls for Symplekin / SYMPK (1 products)

Catalog No. Species Pres. Purity   Source  

SYMPK 293T Cell Transient Overexpression Lysate(Denatured)
SYMPK 293T Cell Transient Overexpression Lysate(Denatured)
  Abnova Taiwan Corp.

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Click here to see all secondary antibodies for Symplekin / SYMPK
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