Coagulation factor VIII (F8) - BM5131

Product Description for Coagulation factor VIII (F8)

Mouse anti Human Coagulation factor VIII (F8) MH104.
Presentation: Purified
Product is tested for Frozen Sections, Enzyme Immunoassay, Western blot / Immunoblot.

Properties for Coagulation factor VIII (F8)

Product Category	Primary Antibodies
Quantity	0.5 mg
Synonyms	AHF, Antihemophilic factor, F8C, Procoagulant component
Presentation	Purified
Reactivity	Hu
Applications	C, E, WB
Clonality	Monoclonal
Clone	MH104
Host	Mouse
Isotype	IgG2a
Shipping to	Worldwide
PDF datasheet	View Datasheet
Manufacturer	OriGene Technologies GmbH
Material safety datasheet	MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen	Swiss Prot Num: P00451 Immunogen: Human factor VIII antigen GeneID: 2157
Isotype control	AM03096PU-N
Application	ELISA: 1:5,000; detection and quantitation of human factor VIII. Immunoblotting. Immunohistochemistry on frozen sections.
Background	Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. It is an extracellular factor. Defects in F8 are the cause of hemophilia A (HEMA). HEMA is a common recessive X linked coagulation disorder. The frequency of hemophilia A is 1-2 in 10,000 male births in all ethnic groups. About 50% of patients have severe hemophilia A with F8C activity less than 1% of normal; they have frequent spontaneous bleeding into joints, muscles and internal organs. Moderately severe hemophilia A occurs in about 10% of patients; F8C activity is 2-5% of normal, and there is bleeding after minor trauma. Mild hemophilia A, which occurs in 30-40% of patients, is associated with F8C activity of 5-30% and bleeding occurs only after significant trauma or surgery. Of particular interest for the understanding of the function of F8C is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8C in their plasma (at least 30% of normal), but the protein is nonfunctional; i.e., the F8C activity is much less than the plasma protein level. CRM reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
General Readings	Stel HV, van der Kwast TH, Veerman EC: Detection of factor VIII / coagulant antigen in human liver tissue. Nature 303, 530-532 (1983)
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Format	Purification: Protein A affinity chromatography Buffer System: PBS, pH 7.4 containing 0.09 % NaN3, 0.5% BSA State: Lyophilized purified IgG Purified Reconstitution: Restore in 1 ml dist. water
Specificity	Specificity: MH104 is specific for human factor VIIIc, a 300 kD protein present in plasma in a complex with von Willebrandt factor. Involved in the clotting cascade (activated by thrombin) by forming a complex with factor IXa, calcium, and phospholipids. Elevated levels of factor VIII have been associated with acute and chronic liver diseases, vascular disorders, diabetes and with acutephase reactions. Species: Human.
Gene ID	2157

Accessory Products

Proteins and/or Positive Controls

Proteins for Coagulation factor VIII (F8) (17 products)

Catalog No.	Species	Pres.	Purity	Source
AR10286PU-L	Coagulation factor VIII (F8)
	Human	Purified
					OriGene Technologies GmbH
AR10286PU-N	Coagulation factor VIII (F8)
	Human	Purified
					OriGene Technologies GmbH
AR10286PU-S	Coagulation factor VIII (F8)
	Human	Purified
					OriGene Technologies GmbH
AR10287PU-L	Coagulation factor VIII (F8)
	Human	Purified	> 97.0% as determined by SDS-PAGE.	CHO cells
					OriGene Technologies GmbH
AR10287PU-N	Coagulation factor VIII (F8)
	Human	Purified	> 97.0% as determined by SDS-PAGE.	CHO cells
					OriGene Technologies GmbH
AR10287PU-S	Coagulation factor VIII (F8)
	Human	Purified	> 97.0% as determined by SDS-PAGE.	CHO cells
					OriGene Technologies GmbH
PA1292	Coagulation factor VIII (F8)
	Human	Purified	> 98 % purified by proprietary chromatographic techniques, purity is greater than 98.0 % as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.	Plasma
					OriGene Technologies GmbH
PA1292X	Coagulation factor VIII (F8)
	Human	Purified	> 98 % purified by proprietary chromatographic techniques, purity is greater than 98.0 % as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.	Plasma
					OriGene Technologies GmbH
PA1292XC	Coagulation factor VIII (F8)
	Human	Purified	> 98 % purified by proprietary chromatographic techniques, purity is greater than 98.0 % as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.	Plasma
					OriGene Technologies GmbH
PA1293	Coagulation factor VIII (F8)
	Human	Purified	> 98 % Purified by proprietary chromatographic techniques, purity is reater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.	CHO cells
					OriGene Technologies GmbH
PA1293X	Coagulation factor VIII (F8)
	Human	Purified	> 98 % Purified by proprietary chromatographic techniques, purity is reater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.	CHO cells
					OriGene Technologies GmbH
PA1293XC	Coagulation factor VIII (F8)
	Human	Purified	> 98 % Purified by proprietary chromatographic techniques, purity is reater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.	CHO cells
					OriGene Technologies GmbH
H00002157-P01	Coagulation factor VIII (F8)
	Human
					Abnova Taiwan Corp.
H00002157-P01-10	Coagulation factor VIII (F8)
	Human	Purified
					Abnova Taiwan Corp.
H00002157-P01-25	Coagulation factor VIII (F8)
	Human	Purified
					Abnova Taiwan Corp.
H00002157-Q01-10	Coagulation factor VIII (F8)
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.
H00002157-Q01-25	Coagulation factor VIII (F8)
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.
					Abnova Taiwan Corp.

Positive controls for Coagulation factor VIII (F8) (3 products)

Catalog No.	Species
H00002157-T01	F8 293T Cell Transient Overexpression Lysate(Denatured)

		Abnova Taiwan Corp.
LY402745	F8 overexpression lysate

		OriGene Technologies, Inc.
NBL1-10413	Factor VIII Lysate

		Novus Biologicals Inc.

Recommended Secondary Antibodies for Coagulation factor VIII (F8) (11 products)

Catalog No.	Host	Pres.	Applications
R1253AP	Mouse IgG (H+L chain) antibody
	Rabbit	AP	C, E, WB
				OriGene Technologies GmbH
R1253B	Mouse IgG (H+L chain) antibody
	Rabbit	Biotin	C, E, WB
				OriGene Technologies GmbH
R1253F	Mouse IgG (H+L chain) antibody
	Rabbit	FITC	E, ICC/IF
				OriGene Technologies GmbH
R1253HRP	Mouse IgG (H+L chain) antibody
	Rabbit	HRP	C, E, WB
				OriGene Technologies GmbH
R1253TR	Mouse IgG (H+L chain) antibody
	Rabbit	Texas Red	ICC/IF
				OriGene Technologies GmbH
R1403R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Rabbit	PE	F
				OriGene Technologies GmbH
R1405R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Goat	PE
				OriGene Technologies GmbH
R1455R	Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed antibody
	Donkey	PE
				OriGene Technologies GmbH
R1457C2	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy2
				OriGene Technologies GmbH
R1457C3	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy3
				OriGene Technologies GmbH
R1457C5	Mouse IgG (H+L chain), adsorbed antibody
	Goat	Cy5
				OriGene Technologies GmbH

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BM5131 Coagulation factor VIII (F8) antibody

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Product Description for Coagulation factor VIII (F8)

Properties for Coagulation factor VIII (F8)

Datasheet Extract

Accessory Products

Proteins and/or Positive Controls

Proteins for Coagulation factor VIII (F8) (17 products)

Positive controls for Coagulation factor VIII (F8) (3 products)

Recommended Secondary Antibodies for Coagulation factor VIII (F8) (11 products)

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