BM5534 alpha skeletal muscle Actin / ACTA1 antibody

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1 ml / €380.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

Quick Overview

Mouse anti Chicken, Human, Mouse, Rat alpha skeletal muscle Actin / ACTA1 647

BM5534

Product Description for alpha skeletal muscle Actin / ACTA1

Mouse anti Chicken, Human, Mouse, Rat alpha skeletal muscle Actin / ACTA1 647.
Presentation: Supernatant
Product is tested for Frozen Sections, Immunocytochemistry/Immunofluorescence, Western blot / Immunoblot.

Properties for alpha skeletal muscle Actin / ACTA1

Product Category Primary Antibodies
Quantity 1 ml
Presentation Supernatant
Reactivity Chk, Hu, Ms, Rt
Applications C, ICC/IF, WB
Clonality Monoclonal
Clone 647
Host Mouse
Isotype IgM
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH
Material safety datasheet MSDS for Monoclonal Antibodies (de)

Datasheet Extract

Immunogen
Swiss Prot Num:
P68133
Immunogen:
Antigen isolated from Chicken skeletal muscle cells
GeneID:
58
Application Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Immunofluorescence.
Immunoblotting: One single band is reactive corresponding to Actin. 
Working Dilution: 1/5-1/10. Dilute immediately before use with PBS or TBS.
Recommended Positive Control: Muscle or myofibrils.
Background The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.
General Readings
  1. Kaehn K, Bachmann P, Falkenberg FW. Immunofluorescence staining of thin-filament sections not participating in actomyosin crossbridges: studies by use of a monoclonal antibody specific to actin. Cell Tissue Res. 1985;239(2):417-22. PubMed PMID: 3884165.
Storage Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Format
Preservatives:
0.09% Sodium Azide
State:
Culture Supernatant
Supernatant
Specificity
Specificity:
This Monoclonal antibody 647 is useful for studying the intracellular distribution and structure of Actin in the cytoskeletal system.
Species:
Human, Mouse, Rat, Chicken.
Gene ID 58

Accessory Products

Proteins and/or Positive Controls

Proteins for alpha skeletal muscle Actin / ACTA1 (4 products)

Catalog No. Species Pres. Purity   Source  

alpha skeletal muscle Actin / ACTA1

Lane 1,9: Phosphorylase B (a), BSA (b), Ovalbumin (c). Lane 2,3: Vimentin. Lane 4,6: Desmin. Lane 5: BSA. Lane 7,8: Actin Rabbit Purified > 98 % (determined by SDS gelelectrophoresis) Skeletal muscle
0.25 mg / €270.00
  OriGene Technologies GmbH

alpha skeletal muscle Actin / ACTA1

Lane 1,9: Phosphorylase B (a), BSA (b), Ovalbumin (c). Lane 2,3: Vimentin. Lane 4,6: Desmin. Lane 5: BSA. Lane 7,8: Actin Rabbit Purified > 98 % (determined by SDS gelelectrophoresis) Skeletal muscle
0.1 mg / €220.00
  OriGene Technologies GmbH

alpha skeletal muscle Actin / ACTA1

alpha skeletal muscle Actin / ACTA1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.

alpha skeletal muscle Actin / ACTA1

alpha skeletal muscle Actin / ACTA1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
  Abnova Taiwan Corp.
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