DA3549S Thrombopoietin

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2 µg / €150.00
Please visit the country specific website of OriGene Technologies or contact your local Distributor to buy this product.

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Thrombopoietin

DA3549S

Product Description for Thrombopoietin

Thrombopoietin.
Presentation: Purified

Properties for Thrombopoietin

Product Category Proteins & Growth Factors
Quantity 2 µg
Synonyms C-mpl ligand, MGDF, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, THPO
Presentation Purified
Molecular weight 18.6 kDa
Source E. coli
Species (Protein) Human
Shipping to Worldwide
PDF datasheet View Datasheet
Manufacturer OriGene Technologies GmbH

Datasheet Extract

Immunogen
Swiss Prot Num:
P40225
Purity > 98 % pure by SDS-PAGE
Add. information Centrifuge vials before opening!
Background Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets.
Storage Lyophilized TPO although stable at Room Temperature for 3 weeks, should be stored desiccated below -18°C.
Upon reconstitution Human TPO should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. 
Avoid repeated freeze-thaw cycles!
Shelf life: One year from despatch.
Description
Description:
Thrombopoietin Human Recombinant produced in E.Coli is a single, non-glycosylated soluble polypeptide chain containing 174 amino acids and having a Molecular Mass of 18608 Dalton which comprises the receptor binding domain of the Mpl-ligand protein.
AA Sequence:
SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNEL
Biological activity:
The ED50 as determined by the dose-dependent stimulation of MO7e cells (with SCF) is 1,95 ng/ml, corresponding to a specific activity of 5 x 105 U/mg.
Molecular weight:
(174 aa)
Specific activity:
5 x 105 units/mg
Format
Endotoxin level:
< 0.1 ng per µg (IEU/µg) of rh TPO
Purity:
>98% pure by SDS-PAGE
State:
Lyophilized (freeze-dried) without buffer and stabilizer
Purified
Reconstitution:
Restore in sterile water not less than 0.1 mg/ml which can then be further diluted into other aqueous buffers.
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